| 长白山四种赤杨丛枝菌根真菌侵染多样性的巢式PCR-RFLP分析 |
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| 引用本文： | 董甜,张惠文,张粤,何兴元.长白山四种赤杨丛枝菌根真菌侵染多样性的巢式PCR-RFLP分析[J].应用生态学报,2006,17(10):1796-1800. |
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| 作者姓名： | 董甜 张惠文 张粤 何兴元 |
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| 作者单位： | 1. 中国科学院沈阳应用生态研究所,沈阳,110016;中国科学院研究生院,北京,100039
2. 中国科学院沈阳应用生态研究所,沈阳,110016 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);中国科学院知识创新工程项目 |
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| 摘 要： | 采集中国吉林长白山不同海拔的4种赤杨根须样本，利用巢式PCR-RFLP方法检测丛枝菌根真菌(AMF)对样品的侵染情况，PCR结果经限制性内切酶分析. 结果表明，赤杨根内AMF存在丰富的基因多样性.AMF的侵染有从宿主混乱性向宿主专一性发展的趋势.东北赤杨AMF的宿主专一性水平最强，球囊霉属已成为东北赤杨的优势侵染类群；其余3种赤杨的AMF则出现宿主混乱现象.宿主因素比海拔因素对AMF侵染有更重要的影响.
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| 关 键 词： | 赤杨 巢式PCR-RFLP 丛枝菌根真菌(AMF) 多样性 长白山 |
| 文章编号： | 1001-9332(2006)10-1796-05 |
| 收稿时间： | 2005-11-15 |
| 修稿时间： | 2006-07-25 |
Genetic diversity of arbuscular mycorrhizal fungi associated with four Alnus species in Changbai Mountains: A nested PCR-RFLP analysis |
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| DONG Tian,ZHANG Huiwen,ZHANG Yue,HE Xingyuan.Genetic diversity of arbuscular mycorrhizal fungi associated with four Alnus species in Changbai Mountains: A nested PCR-RFLP analysis[J].Chinese Journal of Applied Ecology,2006,17(10):1796-1800. |
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| Authors: | DONG Tian ZHANG Huiwen ZHANG Yue HE Xingyuan |
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| Affiliation: | 1.Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | In this paper,the colonization of arbuscular mycorrhizal fungi(AMF)on the root samples of 4 Alnus species in Changbai Mountains was investigated by using nested PCR-RFLP technique,and the PCR results were tested by restriction endonuclease analysis method.The results revealed that the uncultured AMF had a high genetic diversity,and the colonization had a trend from promiscuity to specialization.The AMF from Alnus mandshuica showed the greatest specialization to the host,and Glomus was the dominant colonizer of A.mandshuica.The AMF from A.sibirica var.hirsuta,A.sibirica,and A.tinctoria showed promiscuity,and host had more significant effects on the colonization of AMF than altitude. |
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| Keywords: | Alnus Nested PCR-RFLP arbuscular mycorrhizal fungi Diversity Changbai Mountains |
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