| 悬浮阵列技术在结核分枝杆菌检测中的研究进展 |
| |
| 引用本文： | 孙菲,谢小兵,戴佩希,钟巧青,盛驭.悬浮阵列技术在结核分枝杆菌检测中的研究进展[J].实用预防医学,2016,23(12):1540-1542. |
| |
| 作者姓名： | 孙菲 谢小兵 戴佩希 钟巧青 盛驭 |
| |
| 作者单位： | 1.湖南国际旅行卫生保健中心,湖南 长沙 410100; 2. 湖南中医药大学第一附属医院; 3.河南中医药大学; 4.中南大学湘雅医院 |
| |
| 基金项目： | 国家质检总局科技计划项目(2015IK125)；湖南省科技重点研发计划项目(2015SK2055) |
| |
| 摘 要： | 悬浮阵列技术(Luminex xMAP技术)是一种将编码微球捕获特定检测物的抗原、抗体或核酸探针等分子的有效方法。本文综述了结核分枝杆菌检测的研究进展,探讨以结核分枝杆菌的Rpo B基因和CYP141基因为靶标应用Luminex xMAP技术,构筑悬浮阵列体系,建立一种快速、灵敏、特异的方法,实现结核分枝杆菌及其耐药基因联合检测,拓展出一条更为经济、迅速、便捷的结核分枝杆菌快速检测新路径。
|
| 关 键 词： | 悬浮阵列 结核分枝杆菌 耐药基因 联合检测 |
| 收稿时间： | 2016-05-11 |
Research progress in using Luminex xMAP technology in the detection of Mycobacterium tuberculosis |
| |
| SUN Fei,XIE Xiao-bing,DAI Pei-xi,ZHONG Qiao-qing,SHENG Yu.Research progress in using Luminex xMAP technology in the detection of Mycobacterium tuberculosis[J].Practical Preventive Medicine,2016,23(12):1540-1542. |
| |
| Authors: | SUN Fei XIE Xiao-bing DAI Pei-xi ZHONG Qiao-qing SHENG Yu |
| |
| Affiliation: | Hunan International Travel Healthcare Center, Changsha, Hunan 410100, China |
| |
| Abstract: | Luminex xMAP technology is an effective method for capturing the antigen, antibody, or nucleic acid probe of the specific detection with encoding microbeads. This article reviews the research progress in the detection technology of Mycobacterium tuberculosis and explores the development of a Luminex xMAP system by detecting marker genes,Rpo B and CYP141and the establishment of a fast, sensitive and specific method which can jointly detect Mycobacterium tuberculosis and its resistance genes, and expands a more economical and convenient new way to rapidly detect Mycobacterium tuberculosis. |
| |
| Keywords: | Luminex xMAP Mycobacterium tuberculosis Drug resistance gene Combined detection |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《实用预防医学》浏览原始摘要信息 |
|
点击此处可从《实用预防医学》下载全文 |
|
