| 灰葡萄孢毒素的组分分析和生物测定 |
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| 引用本文： | 郑晓莲,董金皋.灰葡萄孢毒素的组分分析和生物测定[J].植物病理学报,1998,28(3):269-274. |
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| 作者姓名： | 郑晓莲 董金皋 |
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| 作者单位： | 河北农业大学植保系, 保定 071001 |
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| 摘 要： | 用PD培养液培养灰葡萄孢菌,过滤液中含活性成分,经氯仿提取后TLC分离获6种组分,Rf值分别为Ⅰ(0.36)、Ⅱ(0.48)、Ⅲ(0.54)、Ⅳ(0.65)、Ⅴ(0.11)、Ⅵ(0.77),经针刺果实生物测定发现,Ⅲ、Ⅴ、Ⅵ具有毒性。BC-毒素具有一定的广谱性,对不同科属的番茄、茄子、黄瓜、白菜、油菜、小麦、玉米、棉花等都具有不同程度的毒害作用。
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| 关 键 词： | 灰葡萄孢 致病毒素 毒性组分 生物测定 |
ANALYSIS OF TOXICITY COMPOSITION AND BIOASSAY OF BC TOXIN FROM BOTRYTIS CINEREA |
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| Zheng Xiaolian,Dong Jingao,Qi qiusuo,Li Dongmei.ANALYSIS OF TOXICITY COMPOSITION AND BIOASSAY OF BC TOXIN FROM BOTRYTIS CINEREA[J].Acta Phytopathologica Sinica,1998,28(3):269-274. |
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| Authors: | Zheng Xiaolian Dong Jingao Qi qiusuo Li Dongmei |
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| Affiliation: | Hebei Agricultural University, Baodong 171001 |
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| Abstract: | Botrytis cinerea was cultured on PD medium for 21 days, and culturefiltrates contained activity components. The crude BC toxin form chloroformextraction was separated into six separates by TL chromatography,ie. Ⅰ(Rf 0.36) Ⅱ(Rf 0.48) Ⅲ(Rf 0.54) Ⅳ(Rf 0.65) Ⅴ(Rf 0.11)Ⅵ(Rf 0.77),among them, Ⅲ、Ⅴ、Ⅵ had toxicity by fruit bioassay.The experimental results also showed that BC toxin had toxicity to many plants, such as,tomato, eggplant,cucumber,chinese cabbage, rape,corn,wheat,cotton and so on. |
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| Keywords: | Botrytis cinerea Pathotoxin Bioassay Toxicity component |
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