纳米生物传感器在氯霉素检测中的应用

利用pH 敏感的荧光指示剂F1300 可以量化ATP 合酶活性的特点，构建生物传感器，开发灵敏、特异、快速的氯霉素残留检测方法。将pH 变化敏感荧光物质F1300 标记到色素体(chromatophore)的内表面，然后，将β亚基抗体- 生物素- 链亲和素- 生物素- 氯霉素抗体系统连接到色素体上F0F1-ATPase 的β亚基上，该检测体系能与氯霉素相连接。采用微弱发光检测仪检测荧光值，根据荧光值确定氯霉素浓度。样品中氯霉素浓度越高表现出的总荧光值越低，反之亦然。该方法的检测限为1 × 10 － 11mg/mL，且检测操作仅需35min，能快速、高灵敏地检测氯霉素，具有很好的应用前景。

Application of Nano-biosensor to Chloramphenicol Detection

Chloramphenicol is a broad-spectrum antibiotic and its use is prohibited in many countries in the world due to its toxicity and side effects. The detection of chloramphenicol residue in food products has become a critical technical barrier. F1300, a fluorescence indicator, makes it easy to quantify the activity of F0F1-ATPase. Based on this, a complex of chloramphenicol antibody-biotin-streptavidin-biotin- β subunit antibody was constructed and connected to β subunits of F0F1-ATPase in the chromatophore. The concentration of chloramphenicol was measured according to the relative fluoresent value of F0F1-ATPase biosensor tagged by F1300. The detection limit of this method was 1 × 10-11 mg/mL. Only 35 min was needed for the whole detection process. Therefore, this method, thanks to its rapidity, simplicity and high sensitivity, will has a promising application to chloramphenicol detection.