过氧化物酶体增殖物激活受体γ在垂体腺瘤中的表达

目的 通过检测过氧化物酶体增殖物激活受体γ(PPARγ)在人垂体腺瘤组织中的表达,探讨其在垂体腺瘤中的相关机制.方法 通过免疫组化法确定经手术切除的83例垂体腺瘤组织细胞来源;采用RT-PCR、适时定量PCR检测研究83例垂体腺瘤组织及6例正常垂体组织PPARγ的mRNA表达量,采用Western blot法检测组织PPARγ蛋白质表达水平,分析不同类型垂体腺瘤组织之间核酸及蛋白水平表达量的差异.结果 GH腺瘤17例、PRL腺瘤15例、ACTH腺瘤18例、多激素腺瘤(MCPAs)17例、无功能腺瘤(NFAs)16例及正常对照组6例;mRNA水平检测显示所有垂体腺瘤组织的表达量均高于正常对照组,其中GH腺瘤的表达量最高,与其他组比较P＜0.05;蛋白水平检测显示GH腺瘤、PRL腺瘤、ACTH腺瘤及MCPAs的表达量均高于正常对照组(P＜0.05),GH腺瘤的表达量最高,NFAs的表达量与正常对照组差异无统计学意义(P＞0.05).结论 PPARγ转录及蛋白表达水平在人GH、PRL、ACTH及MCPAs垂体腺瘤组织中高表达,该基因与垂体腺瘤有一定的相关性,而无功能垂体腺瘤在核酸水平的表达量增高,蛋白水平的表达量不高,可能与激素的分泌水平有关;在GH腺瘤中的表达量最高,且与其他组相比,差异有统计学意义(P＜0.05),说明该类肿瘤与PPARγ的关系最为密切,可能与生长激素能刺激PPARγ的表达有关.

Correlation of peroxisome proliferation-activated receptor in pituitary adenoma

Objective To prove the expression of PPARγ in human pituitary adenomas,and administrate that the expression among different tumors has significant difference.Method 83 cases of pituitary adenoma tissues and 6 normal pituitary tissues were divided into different groups by immunohischemieal according to the source of tissues and cells.The mRNA of PPARγ in 83 pituitary adenomas tissues and 6 normal pituitary tissues were determined by RT-PCR and quantitative real-time PCR,the protein of PPARγ were detected by western-blot.Result we have 89 samples,17 samples tested as GH secreting-adenomas,15 samples tested as PRL secreting-adenomas,18 samples tested as ACTH secreting-adenomas,17 samples tested as miscellaneous pituitary adenomas (MCPAs),16 samples tested as nonfunctional pituitary adenomas (NFAs) and 6 samples tested as normal;the PPARγ mRNA expressions were higher in all kinds of pituitary adenomas than in the normal pituitary tissue.GH secreting-adenomas was the highest,and the differentia was significant in statistics;the protein expression level of PPARγ in GH secreting,Prolactin secreting,ATCH secreting and MCPAs were higher than in the normal pituitary tissue (P＜0.05),and the expression in GH secreting-adenomas was the highest which has markedly differentia.The expression level of NFAs has no different compared with normal pituitary (P＞0.05).Conclusion We supposed that PPARγ is an important molecular target in pituitary adenoma cells,especially for GH secreting-adenomas.