大黄水提物与醇提物对实验性高脂血症小鼠血脂的影响研究

目的 探讨大黄水提物与醇提物单味应用与1:1伍用对实验性高脂血症小鼠血清中血脂的影响.方法 选取体质量20~25 g昆明种雄性小鼠给予不同方法灌胃,分为对照组、模型组、实验1组(水提取)、实验2组(醇提取)、实验3组(水提取与醇提取1:1配伍),实验28 d后检测各组小鼠血清中三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平.结果 实验1组小鼠TG、LDL-C水平低于模型组(P<0.05),HDL-C高于模型组(P<0.05);实验2组小鼠TC、TG、LDL-C水平低于模型组(P<0.05),HDL-C高于模型组(P<0.05);与实验1组比较,实验2组小鼠 TG、LDL-C降低(P<0.05);与实验2组和实验1组比较,实验3组小鼠TC、TG、LDL-C均降低(P<0.05),HDL-C升高(P<0.05).结论 大黄用乙醇提取降血脂效果较好。

Study on effect of rhubarb water extract and ethanol extract on blood fat in experimental hyperlipidemia mice

Objective To investigate the effect of single use and 1:1 combination use of rhubarb water extract and ethanol extract on blood fat in experimental hyperlipidemia mice.Methods Kunming male mice weighing 20-25 g were selected,given dif-ferent methods of gavage and divided into the control group(Con),model group(Mod),test 1 group(Rhe1,water extract),test 2 group(Rhe2,ethanol extract),test 3 group(water extract and ethanol extract 1:1 compatibility group).The levels of serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)in each group were detected on 28 d after experiment.Results The TG and LDL-C levels in the test 1 group were lower than those in the model group(P<0.05),the HDL-C level was higher than that in the model group(P<0.05);the levels of TC,TG and LDL-C in the test 2 group were lower than those in the model group,the HDL-C level was higher than that in the model group(P>0.05);compared with the test 1 group,the levels of TG and LDL-C in the test 2 group were decreased(P<0.05);compared with the test 2 group and test 1 group,the levels of TC,TG and LDL-C in the test 3 group were decreased and the HDL-C level was in-creased(P<0.05).Conclusion Using ethanol for extracting rhubarb has better effect for reducing blood fat.