海带多糖在高脂血症大鼠中的降血脂和抗氧化作用

目的 探讨海带多糖对高脂血症大鼠血脂的调节作用机制. 方法 健康雌性Wistar大鼠32只,应用高脂饲料喂养方法建立高脂血症动物模型,海带多糖干预治疗.血生化法检测大鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平,硝酸还原酶法检测血清和肝组织一氧化氮(NO)含量,黄嘌呤氧化酶法测定血清和肝组织超氧化物歧化酶(SOD)活性,酶联免疫吸附法检测血清和肝组织中氧化低密度脂蛋白(ox-LDL)水平,免疫组织化学技术检测肝细胞SOD和诱导性一氧化氮合酶(iNOS)表达. 结果 海带多糖治疗后,大鼠血清TG、TC和LDL水平较模型组显著下降,而HDL显著升高(P<0.05).肝组织匀浆ox-LDL和NO水平较模型组显著降低,而SOD活性显著升高.肝细胞iNOS表达较模型组显著降低,SOD表达显著增强(P<0.05). 结论 海带多糖可通过抗氧化机制而发挥调节血脂水平的作用.

Effects of Laminaria japonica polysaccharide to reduce serum lipids and anti-inflammation in hyperlipenmia rats

Objective To investigate the regulating effects and mechanism of Laminaria japonica polysaccharide (LJPS) on lipid levels in serum of hyperlipenmia rats. Methods Thirty two healthy female Wistar rats were used to establish hyperlipemia models by feeding fat rich forage, and treated with LJPS as a potential drug. The serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were determined by biochemical assay. The nitric oxide (NO) level in serum and hepatic tissue was measured by nitrate reductase assay and the activity of superoxide dismutase (SOD) in serum and hepatic tissue was detected by oxidase assay. The level of ox-LDL in hepatic tissue was determined by enzyme-linked immunosorbent assay. The expressions of SOD and inducible nitric oxide synthase (iNOS) in hepatocytes were detected by immunohistochemical assay. Results The rat serum levels of TG, TC and LDL were significantly higher while HDL was lower in the LJPS treated group than those in the hyperlipidemia model group (EM>P/EM><0.05). Both the levels of ox-LDL and NO in both the rat serum and hepatocytic tissues were significantly lower and the activity of SOD was higher in the LJPS-treated group than those in the hyperlipidemia group (EM>P/EM><0.05). The experimental results showed that the iNOS down- expression and the SOD up expression were found in the LJPS-treated group than those in the hyperlipidemia group (EM>P/EM><0.05). Conclusion Laminaria japonica polysacharide- mi