GLUT4在多囊卵巢综合征大鼠子宫内膜中的表达及意义

目的： 探讨葡萄糖转移因子4（GLUT4）在多囊卵巢综合征（PCOS）大鼠子宫内膜中的表达，评价其与子宫内膜胰岛素抵抗(IR)的关系。方法: 54只85日龄SD雌性大鼠，随机分为：① 对照组（20只）；② PCOS组（17只）；③ 二甲双胍治疗组（17只）。其中后两组先参照Poretsky等的方法复制PCOS大鼠模型，造模成功后，再分别喂服安慰剂或二甲双胍， 14 d后断头处死各组大鼠并取材。采用免疫组织化学染色Elivision^TM Plus两步法检测对照组、PCOS组及治疗组大鼠子宫内膜GLUT4蛋白的表达。结果: PCOS组大鼠子宫内膜腺上皮中GLUT4和INS-R蛋白表达明显低于对照组（P＜0.01，P＜0.05），INS蛋白表达水平明显高于对照组水平（P＜0.01）；治疗组GLUT4表达显著高于PCOS组（P＜0.01），但仍低于对照组（P＜0.01），INS表达较PCOS组显著降低（P＜0.05），但仍高于对照组（P＜0.05）；子宫内膜间质中无GLUT4的表达，INS和INS-R表达情况与腺上皮相似。结论: PCOS大鼠子宫内膜GLUT4表达减少和子宫内膜的IR有关。

Expression of GLUT4 in endometrium of rats with polycystic ovary syndrome

AIM: To explore the expression of glucose transporter 4 (GLUT4) in the endometrium of rats with polycystic ovarian syndrom (PCOS) and evaluate the relationship between GLUT4 expression and insulin resistance (IR). METHODS: 54 female SD rats of 85 days were randomized to control group (n=20), PCOS model group (n=17) and metformin treatment group (n=17). The rats in the latter two groups were induced by Poretsky's method for PCOS model, followed by placebo or metformin, respectively. After 14 days of treatment, the rats were sacrificed and the expression of GLUT4 in endometrium was detected by ElivisionTM Plus two steps immunohistochemical staining. RESULTS: The expression of GLUT4 and insulin receptor(INS-R) proteins of endometrial glandulan epitheliu in PCOS rats were significantly lower (P<0.01,P<0.05) than those in control group, however, the expression of insulin(INS) protein in PCOS rats was higher than that in control group (P<0.01). The expression of GLUT4 in the treatment group increased (P<0.01), but was still lower than that in control group (P<0.01). However, compared with PCOS group, the expression of INS protein was decreased (P<0.05), but was still higher than that in control group (P<0.05). There was no GLUT4 expression in interstitial cells in endometrium, and the changes of the expressions of INS and INS-R proteins in those cells were similar with those in glandulan epitheliu. CONCLUSION: The decrease in GLUT4 expression of endometrium in PCOS rats is related with endometrial insulin resistance.