苓枣片中酸枣仁皂苷A和细叶远志皂苷的含量测定

目的:测定中药苓枣片中酸枣仁皂苷A和细叶远志皂苷的含量.方法:应用HPLC-ELSD法检测酸枣仁皂苷A的含量,采用DIONEX Acclaim 120 C18色谱柱,以乙腈-水为流动相梯度洗脱,ELSD检测雾化温度40 ℃,压力3.6 bar;采用HPLC检测细叶远志皂苷的含量,流动相为甲醇-0.05%磷酸溶液,检测波长210 nm.结果:酸枣仁皂苷A在0.1～1.0 mg·ml-1 内呈良好的线性关系,平均回收率为98.77%;细叶远志皂苷在0.0625～1.0000 mg·ml-1内呈良好的线性关系,平均回收率为98.47%.结论:该方法简便、灵敏、准确、重复性好,可用于苓枣片的质量控制.

Determination of Jujuboside A and Tenuifolin in Lingzao Tablets

Objective:To determine the content of jujuboside A and tenuifolin in Lingzao tablets.Method:HPLC-ELSD was used to determine the content of jujuboside A.A DIONEX Acclaim 120 C₁₈ column was applied with mobile phase consisting of acetonitrile and water with gradient elution,and the temperature and pressure of ELSD detector was set at 40℃and 3.6 bar.Tenuifolin was determined with methanol-0.05%phosphoric acid as the mobile phase and the detection wavelength was set at 210 nm.Result:The linear range of jujuboside A was 0.1-1.0 mg ? ml^-1,and the average recovery was 98.77%.The linear range of tenuifolin was 0.062 5- 1.000 0 mg ? ml^-1,and the average recovery was 98.47%.Conclusion:The methods are simple,sensitive,accurate and reproducible for the determination of jujuboside A and tenuifolin in Lingzao tablets.