蛋白酪氨酸磷酸酶1B基因位点IVS6＋G82A多态性与肥胖相关性高血压研究

目的探讨PTP1B基因位点IVS6＋G82A多态性与肥胖高血压的相关性。方法选取来自上海、南京地区汉族高血压患者393例（其中男性210例,女性183例;年龄25～70岁,平均年龄56.59岁）和非高血压患者266例（其中男性141例,女性125例;年龄28～72岁,平均年龄57.03岁）,根据体质量指数（BMI）是否≥24kg/m2分为超质量高血压组242例,单纯高血压组151例,超质量对照组116例,正常对照组150例。运用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法测定PTP1B基因位点IVS6＋G82A的多态性。结果超质量高血压组GG基因型及等位基因G频率高于单纯高血压组、超质量对照组和正常对照组。多元逻辑回归分析显示在超质量人群中,位点IVS6＋G82A等位基因G、性别、甘油三酯（TC）、低密度脂蛋白胆固醇（LDL-C）、胰岛素敏感指数是高血压发病的独立相关因素。以AA基因型为参照,GG基因型患肥胖相关性高血压的比值比（OR）为2.29（P〈0.01）。在超质量高血压组中,GG基因型人群血清TC、LDL-C水平高于AA和AG（P〈0.05）基因型人群。结论位点IVS6＋G82A变异与肥胖高血压相关,并与患者脂代谢异常相关。

Protein tyrosine phosphatase 1B gene polymorphisms and obesity-related hypertension:a case-control study in Chinese population

Objective To investigate the relevance of protein tyrosine phosphatase 1B（PTP1B） IVS6 ＋ G82A with obesity-related hypertension in Chinese subjects.Methods A total of 393 Chinese patients with hypertension（male 210,female 183;aged 25-70 years,mean age 56.59 years） and 266 non-hypertensive subjects（male 141,female 125;aged 28-72 years,mean age 57.03 years） were enrolled.According to body mass index（BMI） and blood pressure,all patients were stratified into 4 groups：overweight hypertension group（n = 242;BMI ≥ 24 kg/m2）,lean hypertension group（n = 151;BMI 24 kg/m2）,overweight control group（n = 116;BMI ≥ 24 kg/m2） and lean control group（n = 150;BMI 24 kg/m2）.The genotypes of PTP1B gene polymorphisms of subjects were determined by polymerase chain reaction-restriction fragment length polymorphism methods.Results The case-control study showed the association between the frequencies of GG genetype,G allele of IVS6 ＋ G82A and obesity-related hypertension（P 0.05）.In obese subjects,multivariate logistic regression analysis showed that the GG genetype had higher risks of obesity-related hypertension but independent of gender,lipid profiles and insulin sensitive index（OR：2.29,P 0.01）.In addition,significant associations were observed between the IVS6 ＋ G82A polymorphism and lipid levels in overweight hypertensive patients.But GG genetype was associated with higher plasma TC and LDL-C concentration compared with AA and AG genetype（P 0.05）.Conclusion It is demonstrated that PTP1B gene polymorphisms may contribute to pathogenesis of obesity-related hypertension in Chinese subjects and involve in the development of several metabolic features such as dyslipidemia in these subjects.