| 多重PCR检测转基因水稻的转基因成分 |
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| 引用本文： | 魏霜,陈贞,芦春斌,马骏,白卫滨,吴希阳.多重PCR检测转基因水稻的转基因成分[J].食品科学,2012,33(12):159-162. |
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| 作者姓名： | 魏霜 陈贞 芦春斌 马骏 白卫滨 吴希阳 |
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| 作者单位： | 暨南大学理工学院食品科学与工程系;暨南大学生殖免疫研究所;广东出入境检验检疫局检验检疫技术中心 |
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| 基金项目： | 农业部转基因重大专项(2008ZX08012-005) |
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| 摘 要： | 以水稻内源基因SPS、外源抗虫基因Cry1Ab、外源抗虫基因Cry1Ab/Ac、外源抗虫基因Btc、报告基因GUS、NOS终止子和CaMV35S启动子为检测对象,设计7对引物,通过优化PCR扩增体系中不同引物浓度的配比及退火温度,建立水稻转基因成分的七重PCR检测体系。结果表明:建立的七重PCR体系能有效检测出水稻及其他作物(大豆、玉米、棉花籽、菜籽粕)中的转基因成分,检测过程简便、特异性好。
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| 关 键 词： | 转基因检测 水稻 七重PCR |
Multiplex PCR Detection of Transgenic Components of Genetically Modified Rice |
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| WEI Shuang,CHEN Zhen,LU Chun-bin,MA Jun,BAI Wei-bin,WU Xi-yang.Multiplex PCR Detection of Transgenic Components of Genetically Modified Rice[J].Food Science,2012,33(12):159-162. |
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| Authors: | WEI Shuang CHEN Zhen LU Chun-bin MA Jun BAI Wei-bin WU Xi-yang |
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| Affiliation: | 1,(1.Department of Food Science and Engineering,College of Science and Engineering,Jinan University,Guangzhou 510632,China;2.Institute of Reproductive Immunology,Jinan University,Guangzhou 510632,China;3.Inspection and Quarantine Technology Center,Guangdong Entry-Exit Inspection and Quarantine Bureau,Guangzhou 510623,China) |
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| Abstract: | In order to detect transgenic components in genetically modified rice,endogenous gene sps,exogenous insect resistant gene Cry1Ab/Ac,exogenous insect resistant gene Cry1Ab,exogenous insect resistant gene Btc,reporter gene gus,nos terminator and CaMV35S promoter were evaluated by multiplex polymerase chain reaction.Seven pairs of primers were designed,and primer concentration and annealing temperature were optimized to establish a multiplex PCR system.The results showed that the developed seven-plex PCR system could allow the detection of transgenic components in genetically modified rice and other crops including soybean,corn,cottonseeds and rapeseed meal with high efficiency and good stability. |
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| Keywords: | transgenic detection rice seven-plex PCR |
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