慢性阻塞性肺疾病大鼠模型中瘦素及白介素-8的表达分析

目的：观察COPD大鼠模型中瘦素（1eptin）、白细胞介素8（IL-8）的表达情况，分析其相关性，探讨leptin在COPD发生发展中的作用及意义。方法：36只雄性SD大鼠随机分为①健康对照组；②COPD模型1组：分别于第（1、14）d经气管内注入内毒素200ug，熏5％香烟（第1、14d除外），2h／d，共4周；⑧COPD模型2组：单纯熏5％香烟2h／d，共12周。观察肺组织病理变化，免疫组化法测定leptin、IL-8在支气管肺组织的表达情况，放免法测定血清leptin及IL-8浓度。结果：细胞因子leptin及炎性因子IL-8在支气管肺组织阳性表达。LPS联合熏烟诱导COPDl组支气管肺组织中leptin（52．67±04．72）和IL-8（59．56±3．94）表达较单纯熏烟诱导COPD2组leptin（38．89±2．57）和IL-8（55．22±3．42）表达明显升高，P〈0．05，两组COPD大鼠模型支气管肺组织中leptin及IL-8表达较正常对照组leptin（16．90＋1．52）和IL-8（28．00±4．24）表达均明显增高，P〈0．05，COPD1组血清中leptin（3．26±0．95）ng／mL和IL-8（107．51±13.38）pg／mL较COPD2组中leptin（2．42±0．69）ng／mL和IL-8（（94．07±11．20）pg／mL明显增高，P〈0．05，两组COPD大鼠模型血清中leptin及IL培浓度较正常对照组leptin（0．95±0．56）ng／mL和IL-8（39．48±6．35）pg／mL浓度显著升高，P〈0．05。血清中Leptin与IL-8表达水平呈显著~-ffn关（r值分别为0．720．670．84均P〈0．05）。经过q检验，两两之间比较均有统计学意义。结论：leptin和IL-8均参与cOPD炎症反应过程，并且具有相关性，LPS促进二者的表达。

The Analysis of Leptin and Interleukin-8 Expression in Rat Models of Chronic Obstructive Pulmonary Disease

Objective： To investigate the expression matter of leptin and IL-8 in rat models of COPD, and analyse their relevance, investigate the value of leptin in the development of COPD. Methods： 36 Male SD rats were randomly divided into 3 groups： （1） Control group： untreated rats; （2）COPD 1： rats were intratracheally administered LPS （200 μg） on d 1 and 14 and exposed to 5% smoke for 2 h daily for 4 consecutive weeks; （3） COPD2： rats were exposed to 5% smoke for 2 h daily for 12 consecutive weeks. To observe the pathological changes of the lung tissues, and take immunohistochemistry to determine leptin and IL-8 levels expressed in bronchial lung tissue. Serum leptin and IL-8 concentration was examined by radio-immunity method. Results： The cell factor leptin and inflammatory factor IL-8 in bronchial lung tissue were positive expressed. The expression ofleptin （ 52.67± 04.72）and IL-8 （59.56± 3.94） in COPD 1 group was higher than that in COPD2 group leptin（38.89± 2.57） and IL-8（55.22± 3.42）, P〈0.05; Both leptin and IL-8 expression in two COPD model groups were higher than that in the normal group （16.90± 1.52） and （28.00± 4.24）, P〈0.05; In the COPD1 group, serum leptin level （3.26± 0.95） ng/mL and IL-8（107.51± 13.38） levels were markedly higher than that in the COPD2 group（2.42± 0.69）ng/ml and （94.07 ± 11.20） pg/mL, P 〈 0.05, and these levels in both COPD groups increased dramatically compared with that in controls （0.95± 0.56）ng/mL and IL-8 （39.48± 6.35） pg/mL, P〈0.05. There was negative correlation between leptin and IL-8 expression（r=0.72 0.67 0.84 all P〈0.05）in blood. The comparison between the two groups had statistical significance after inspection. Conclusion： Leptin and IL-8 play important roles in the occurrence and development of COPD, which is correlated with each other. In addition, LPS facilitates the expression of leptin and IL-8.