| 黄蜀葵花期不同组织器官中多类型资源性化学成分的分析与利用价值挖掘 |
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| 引用本文： | 刘杰,郭盛,段金廒,严辉,钱大玮,唐海涛,唐仁茂.黄蜀葵花期不同组织器官中多类型资源性化学成分的分析与利用价值挖掘[J].中国中药杂志,2016,41(20):3782-3791. |
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| 作者姓名： | 刘杰 郭盛 段金廒 严辉 钱大玮 唐海涛 唐仁茂 |
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| 作者单位： | 南京中医药大学 江苏省中药资源产业化过程协同创新中心 国家中医药管理局中药资源循环 利用重点研究室 中药资源产业化与方剂创新药物国家地方联合工程研究中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心 国家中医药管理局中药资源循环 利用重点研究室 中药资源产业化与方剂创新药物国家地方联合工程研究中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心 国家中医药管理局中药资源循环 利用重点研究室 中药资源产业化与方剂创新药物国家地方联合工程研究中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心 国家中医药管理局中药资源循环 利用重点研究室 中药资源产业化与方剂创新药物国家地方联合工程研究中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心 国家中医药管理局中药资源循环 利用重点研究室 中药资源产业化与方剂创新药物国家地方联合工程研究中心, 江苏 南京 210023,江苏苏中药业集团股份有限公司, 江苏 泰州 225500,江苏苏中药业集团股份有限公司, 江苏 泰州 225500 |
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| 基金项目： | 江苏省产学研合作前瞻性联合研究项目（BY2015008-04）；江苏省高校中药学优势学科Ⅱ期建设项目（2014-ysxk） |
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| 摘 要： | 对锦葵科植物黄蜀葵Abelmoschus manihot采收药用部位花冠过程中产生的不同组织器官(根、茎、叶和花)中的主要资源性化学成分进行分析评价,为其资源化利用提供支撑。分别采用高效液相色谱法(HPLC)、超高效液相色谱串联三重四极杆质谱法(UPLC-TQ/MS)分析黄蜀葵不同组织器官中黄酮类、核苷类及氨基酸类资源性化学成分组成及其含量;分别采用紫外-可见分光光度法、粗纤维测定法(Weende)分析黄蜀葵不同组织器官中可溶性多糖类及总纤维含量。结果显示,黄蜀葵花中富含黄酮类资源性化学成分,主要组成为金丝桃苷、异槲皮苷、棉皮素-8-O-葡萄糖醛酸苷、杨梅素、槲皮素-3'-O-葡萄糖苷、芦丁和槲皮素,总量为25.450 mg·g~(-1),叶片亦有少量黄酮类资源性化学成分分布。黄蜀葵不同组织器官均富含可溶性多糖类组分和总纤维,其中茎中总多糖量较高,达19.76%,根中总纤维量较高,可达29.88%。在黄蜀葵植物中共检出21种氨基酸类和9种核苷类资源性化学成分,其中花中氨基酸类化学成分种类及含量较为丰富,达4.737 mg·g~(-1);叶中核苷类化学成分含量较为丰富,为1.474 mg·g~(-1)。黄蜀葵植物中含有丰富的资源性化学成分,且不同组织器官中所含成分的组成和含量有所差异,研究结果为黄蜀葵植物采收花后不同组织器官的精细化利用与产业化开发提供了科学依据。
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| 关 键 词： | 黄蜀葵 不同组织器官 资源性化学成分 资源化利用 |
| 收稿时间： | 2016/7/13 0:00:00 |
Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot |
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| LIU Jie,GUO Sheng,DUAN Jin-ao,YAN Hui,QIAN Da-wei,TANG Hai-tao and TANG Ren-mao.Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot[J].China Journal of Chinese Materia Medica,2016,41(20):3782-3791. |
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| Authors: | LIU Jie GUO Sheng DUAN Jin-ao YAN Hui QIAN Da-wei TANG Hai-tao and TANG Ren-mao |
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| Affiliation: | Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China,SZYY Group Pharmaceutical Ltd., Taizhou 225500, China and SZYY Group Pharmaceutical Ltd., Taizhou 225500, China |
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| Abstract: | This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value. The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS. The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3''-O-glucoside, rutin and quercetin. The total content of these flavonoids is 25.450 mg·g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose. Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg·g-1), while the leaves have the highest content of nucleosides (1.474 mg·g-1). A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants. |
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| Keywords: | Abelmoschus manihot different organs and tissues resourceful chemical composition resourceful utilization |
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