Novel Chitinolytic Enzymes with Biological Activity Against Herbivorous Insects |
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| Authors: | Roxanne M Broadway Carmenza Gongora Wendy C Kain John P Sanderson Jose A Monroy K C Bennett Jason B Warner Michael P Hoffmann |
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| Affiliation: | (1) Department of Entomology, NY State Agricultural Experiment Station, Cornell University, Geneva, New York, 14456;(2) Department of Entomology, Cornell University, Ithaca, New York, 14853 |
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| Abstract: | The soil bacteria, Streptomyces albidoflavus, secretes endochitinases and chitobiosidases that are active over a broad range of pH (4–10). Ingestion of this mixture of chitinolytic enzymes significantly reduced the growth and development of Trichoplusia ni and significantly reduced survival of Myzus persicae, Bemisia argentifolii, and Hypothenemus hampei. Perfusion chromatography was used to separate endochitinases from chitobiosidases. The endochitinases had significantly greater biological activity against Bemisia argentifolii than the chitobiosidases. The utility of chitinolytic enzymes as regulators of populations of herbivorous insects is discussed. |
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| Keywords: | Endochitinase chitobiosidase Trichoplusia ni Bemisia argentifolii Hypothenemus hampei Myzus persicae |
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