人参皂甙诱导HL-60细胞凋亡的研究

目的：观察人参皂甙（GS）是否具有诱导髓性白血病HL－60细胞株的凋亡作用。方法：取不同浓度的GS处理HL－60细胞，观察GS所致的细胞形态学的变化；流式细胞术分析细胞DNH含量的改变，并进行DNA片段分析（DNA Ladder);用Annexin V-FITC试验法分析细胞凋亡百分率。结果：人参皂甙能够抑制HL－60细胞生长，在一定剂量和时间范围内可引起细胞凋亡。结论：提示人参皂甙能特异性地诱导HL－60细胞凋亡，可为临床应用人参皂甙作为化疗药物的辅助剂治疗白血病提供实验依据。

Study on Induction of Ginsenosides on HL-60 Cell Apoptosis

Objective: To observe whether the Ginsenosides (GS) could induce HL 60 cell line apoptosis from promyelocytic leukemia. Methods: HL 60 cells were treated with GS of various concentration to observe the effect of GS on cell morphological change, the DNA content change by flow cytometry, DNA ladder by electrophoresis, and apoptosis rate by Annexin V FITC test of the cells. Results: GS could inhibit the growth of HL 60 cells and induce cell apoptosis in a certain scope of dose and reacting time. Conclusion: GS could specifically induce apoptosis in HL 60 cells, which provides an experimental basis for treatment of leukemia with GS as an supplemntary agent of chemotherapy.