人胎阑尾IAPP-、SS-及5-HT免疫反应性细胞的免疫组织化学研究

为了探讨胰岛淀粉样多肽，在胎阑尾中的个体发及其与其他生物活性物质的关系，用免疫组织ＰＡＰ法，对３６例１２－３８周人胎阑尾中ＩＡＰＰ免疫反应性细胞，生称抑素ＩＲ细胞５－羟色胺－ＩＲ细胞进行定位研究。结果表明，肥１２周，阑尾上皮中已可见到ＳＳ－ＩＲ细胞，而ＩＡＰＰ－及５－ＨＴ－ＩＲ细胞于１４周才见到。在整个胎期，阑尾中的ＩＡＰＰ－及ＳＳ－ＩＲ细胞始络分散存在，数量较少，而５－ＨＴ－ＩＲ细胞数量随胎龄增

IMMUNOHISTOCHEMICAL STUDY OF IAPP-、SS- AND 5-HT-IMMUNOREACTIVE CELLS IN HUMAN FETAL APPENDIX

In order to investigate the ontogeny of the islet amyloid polypeptide (IAPP) and explore the relationship between IAPP and other classical hormones in human fetal appendix, the localization of IAPP immunoreactive (IR) cells, somatostatin (SS) IR cells and 5 hydroxytryptaming (5 HT) IR cells in 36 human fetal appendix from 12 to 38 weeks of gestation was investigated with immunohistochemical PAP method. The results showed that at 12 week of fetal age, the SS IR cells were found in the appendix epithelium, but IAPP and 5 HT IR cells appeared at 14 week. In the whole fetus period, IAPP and SS IR cells appeared fewer and scattered, while the number of 5 HT IR cells changed with the gestation age, showed a peak from 15 to 21 weeks of gestation. As compared with adjacent sections, the co localization of IAPP with SS or 5 HT in the same cell was not found. The results indicated that IAPP,SS and 5 HT had been synthesized in endocrine cells of human fetal appendix.