| 小鼠乳癌细胞辐射敏感突变株SX-9DNA双链断裂及其重接修复研究 |
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| 引用本文： | 周平坤,魏康.小鼠乳癌细胞辐射敏感突变株SX-9DNA双链断裂及其重接修复研究[J].辐射研究与辐射工艺学报,1992(1). |
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| 作者姓名： | 周平坤 魏康 |
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| 作者单位： | 军事医学科学院放射医学研究所 北京
(周平坤)，军事医学科学院放射医学研究所 北京(魏康) |
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| 摘 要： | 本研究采用一种新的细胞DNA双链断裂检测方法——脉冲电场电泳法,检测了两株来源相同而辐射敏感性不同的细胞SR-1和SX-9的X线照射所致DNA双链断裂的产生和修复。结果表明两株细胞的DNA双链断裂产生没有差别,而辐射敏感细胞SX-9的DNA双链断裂重接修复能力明显低于SR-1细胞,本文对此结果与细胞辐射敏感性的关系进行了讨论。
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| 关 键 词： | DNA双链断裂 细胞辐射敏感性 脉冲电场电泳 辐射敏感突变株 |
STUDIES ON DNA DSB AND ITS REJOINING REPAIR IN A MUTATED RADIOSENSITIVE MOUSE MAMMARY CANCER CELL LINE, SX-9 |
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| Zhou Pingkun,Wei Kang Institute of Radiation Medicine Academg of Military Medical Science,Beijing.STUDIES ON DNA DSB AND ITS REJOINING REPAIR IN A MUTATED RADIOSENSITIVE MOUSE MAMMARY CANCER CELL LINE, SX-9[J].Journal of Radiation Research and Radiation Processing,1992(1). |
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| Authors: | Zhou Pingkun Wei Kang Institute of Radiation Medicine Academg of Military Medical Science Beijing |
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| Abstract: | The intinial amount and repair of DNA double strand breaks (DSBs)
in the radiosensitive cell SX-9 and wild-type cell SR-1 irradiated by X ray were
detected by pulsed field gel electrophoresis. The results showed that the extent of DNA
DSBs was similar in these two cell lines under same X ray doses, but the rejoining
repairca pability of DNA DSBs in SX-9 cells was significantly lower than that in SR-1
cells. Results suggested that the increased radiosensitivity in SX-9 cells could be corr-
elated with a defect in the repair of DNA DSBs. |
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| Keywords: | DNA double strand breaks (DSBs) Radiosensitivity Pulsed field gel electrophoresis Radiosensitive mutant |
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