几种营养物质对烟草细胞生长和CoQ10含量的影响

研究了蔗糖、KH₂PO₄、NH₄NO₃比对烟草细胞生长和CoQ₁₀量的影响，结果表明，当蔗糖浓度为30g/L时，CoQ₁₀总量最高，此时细胞产量、CoQ₁₀含量和总量分别为19.8g/L、414.7μg/g(dwt)、8212μg/L。在上述蔗糖浓度下，当KH₂PO₄起始浓度为340mg/L、NH₄NO₃为12时，细胞产量、CoQ₁₀含量和总量分别最高，高于此比例时有利于CoQ₁₀形成 ，但不利于细胞生长。

Effects of A Few Nutritional Factors on Content of Coenzyme Q10 by Tobacco Plant Cells in Suspension Culture

With suspension cultured cells of tobacco (Nicoliana Tobacum L.), effects of inorganic phosphate, nitrogen sources and carbon source on cell growth and CoQ10 content were investigated. It showed that, When treated with 30 g/L of sucrose, the total amount of CoQ10 was highest (8212 g/L); and the cell biomass and CoQ10 content were 19.8 micrograms/L, 414.7 micrograms/g(dwt) respectively. When treated 340 mg/L of KH2PO4, the cell biomass, the content and total amount of CoQ10 were 21.04 g/L, 514.5 micrograms/g(dwt) and 10824 micrograms/L respectively. When treated with the ratio of NH4+/NO3- was 1:2, the cell biomass, the content and total amount of CoQ10 were 21.04 g/L, 514.3 micrograms/g(dwt) and 10,820 micrograms/L respectively. The high ration of NH4+/NO3- was suitable for the formation of CoQ10, but not for the cell growth.