| 高效液相色谱分析银杏叶提取物中总黄酮甙 |
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| 引用本文： | 王爱民.高效液相色谱分析银杏叶提取物中总黄酮甙[J].贵州师范大学学报(自然科学版),1997(3). |
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| 作者姓名： | 王爱民 |
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| 作者单位： | 贵州师大分析测试中心 |
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| 摘 要： | 将银杏叶提取物经酸水解得到其中黄酮甙元槲皮素、山萘酚和异鼠李素，采用高效液相色谱、ＹＷＧ－Ｃ１８柱，甲醇：水（含０４％磷酸）为流动相，ＵＶ３６０ｎｍ检测，以槲皮素为对照品计算出银杏叶提取物中总黄酮甙含量。槲皮素浓度在００１～０２ｍｇ／ｍｌ之间，浓度与峰面积呈直线关系，相关系数为０９９９２，回收率为９７４％，相对标准偏差１６１％。该方法简便、准确，易于银杏叶提取物的质量控制。
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| 关 键 词： | 高效液相色谱，银杏叶提取物，黄酮甙，槲皮素 |
ALANYSIS OF THE FLAVONOIDS FROM GINKGO BILOBA EXTRACT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
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| Wang Aimin.ALANYSIS OF THE FLAVONOIDS FROM GINKGO BILOBA EXTRACT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].Journal of Guizhou Normal University(Natural Sciences),1997(3). |
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| Authors: | Wang Aimin |
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| Abstract: | Hydrolysis of the extracts of the ginkgo biloba L obtains aglycones quercentin, kaempferol and isorhamnetin HPLC method has been uesed in separation and determination of aglycones on YWG-C 18 column with methanol-0 4% phosporic acid as eluents, which has been detected by ultraviolet detector at 360 nm A good linear relationship of quercentin has been found in the range of 0 01-0 20 mg/ml The average recovers are 97 4% The relative standard deviation is 1 61% This method is sample and accurate for quality control of the extracts of the ginkgo biloba L |
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| Keywords: | HPLC Flavoinds Quercetin Extract of ginkgo biloba L |
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