| SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE （11A1） BY TUMOR SUPPRESSOR GENE RB |
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| 引用本文： | 王冬梅,李申德,崔惠云,杨晓洁.SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE （11A1） BY TUMOR SUPPRESSOR GENE RB[J].中国医学科学杂志,1997,12(2):76-79. |
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| 作者姓名： | 王冬梅 李申德 崔惠云 杨晓洁 |
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| 作者单位： | CancerInstitute,CAMS＆PUMC,Beijing100021 |
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| 摘 要： | A malignant transformed mammary epithelial cell line (11 A1) was transfected with liposome encapsulated eukaryotic expression plasrrdd pCMV-neo-RB, yielding 4 constant clones which have obvious phenotypic reversion changes, and named 11Al-R1～R4 respectively. Further experiments showed that the llA1-R1 behaved like normal epithelial cells in both morphological and hiolcgical characteristics, with decreased clonogenicity in solid argar medium as well as decreased tumorigenicity. Northern blot hybridization showed increased expression of RB gene and decreased expression of c myc gene in llA1-R1, 11A1-R2 cells compared to llA1 cells. This was an ideal phenotypic reversion model for epithelial transformed cell line and demonstrated that the RB gene can reexpress and suppress malignant phetlotype in RB inactive cells.
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| 关 键 词： | 肿瘤抑制基因 乳腺上皮细胞 基因转染 动物模型 试验 llA1-R1 |
SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE (11A1)BY TUMOR SUPPRESSOR GENE RB |
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| D Wang,S Li,W Au,H Cui,X Yang.SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE (11A1)BY TUMOR SUPPRESSOR GENE RB[J].Chinese Medical Sciences Journal,1997,12(2):76-79. |
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| Authors: | D Wang S Li W Au H Cui X Yang |
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| Affiliation: | Cancer Institute, CAMS & PUMC, Beijing 100021. |
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| Abstract: | A malignant transformed mammary epithelial cell line (11A1) was transfected with liposome encapsulated eukaryotic expression plasmid pCMV-neo-RB, yielding 4 constant clones which have obvious pheno-typic reversion changes, and named 11A1-R1-R4 respectively. Further experiments showed that the 11A1-R1 behaved like normal epithelial cells in both morphological and biological characteristics, with decreased clonogenicity in solid argar medium as well as decreased tumorigenicity. Northern blot hybridization showed increased expression of RB gene and decreased expression of c-myc gene in 11A1-R1, 11A1-R2 cells compared to 11A1 cells. This was an ideal phenotypic reversion model for epithelial transformed cell line and demonstrated that the RB gene can reexpress and suppress malignant phenotype in RB inactive cells. |
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| Keywords: | RB gene phenotypic reversion transformed cell line |
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