沙漠枯草芽孢杆菌 PJS 产生的环脂肽类抗生素研究

目的 分离鉴定枯草芽孢杆菌PJS发酵液中的环脂肽类抗生素PJS-0。方法 采用溶剂萃取、薄层色谱（TLC）、高效液相色谱法（HPLC）等分离方法 对菌株PJS发酵产物的活性组分PJS-0进行分离；并通过紫外（UV）、质谱（MS）和核磁共振波谱（NMR）联合分析对其结构进行鉴定。结果 从塔克拉玛干沙漠来源植物内生枯草芽孢杆菌PJS的发酵液中分离得到一个环脂肽类抗生素：PJS-0，经结构鉴定为Mojavensin A。结论 本文首次从陆生芽孢杆菌中分离得到Mojavensin A，并首次用ESI^＋-MS／MS对其氨基酸序列进行鉴定。

Study on the cyclic lipopeptide group antibiotic produced by Bacillus subtilis strain PJS from desert

Objective To isolate and identify compound PJS-0 from the fermentation broth produced by Bacillus subtilis strain PJS. Methods Compound PJS-0 was isolated and purified by using solvent extraction, TLC and HPLC. UV, MS and NMR were analyzed to elucidate the structure of PJS-0. Results Compound PJS-0 was obtained from the fermentation broth produced by Bacillus subtilis strain PJS isolated from Taklamakan Desert, and structurally identified as Mojavensin A, acyclic lipopeptide group antibiotic. Conclusion Mojavensin A is discovered from the fermentation broth of terrestrial-derived Bacillus subtilis strain PJS for the first time and amino acid sequence of Mojavensin A is also analyzed by the tandem mass spectrometry method （ESI^＋-MS/MS） for the first time.