The differentiation of HL-60 cells in the synthetic medium induced by GM3 ganglioside |
| |
| Authors: | Haruki Senoo Takashi Momoi |
| |
| Affiliation: | (1) Department of Anatomy, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113 Tokyo, Japan;(2) Division of Chemical Pathology, National Center fro Nervous, Mental and Muscular Disorders, 4-1-1 Ogawahigashi-machi, Kodaira, 187 Tokyo, Japan |
| |
| Abstract: | GM3 ganglioside, added exogenously to a promyelocytic leukemia cell line (HL-60 cells) in serum-free synthetic medium, induced differentiation into macrophage-like cells. Macrophagic morphology and function of differentiation-induced cells were determined by cell growth behavior, May-GriJnwald-Giemsa staining, activities of nonspecific esterase, phagocytosis and nitroblue tetrazolium (NBT) reduction. GM3 ganglioside may play a role in triggering differentiation of HL-60 cells into macrophage-like cells. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
