| Preparation,purification and characterization of the polyclonal antibody against α1-AT |
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| 引用本文： | 王彩霞,朱建思,周见远,冯惊涛,赵辉,邓海欧,吴小晋,聂东宋.Preparation,purification and characterization of the polyclonal antibody against α1-AT[J].国际生物制品学杂志,2003,32(1):122-124. |
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| 作者姓名： | 王彩霞 朱建思 周见远 冯惊涛 赵辉 邓海欧 吴小晋 聂东宋 |
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| 作者单位： | 南华大学肿瘤研究所,衡阳,421001;湖南景达生物工程有限公司科研办公室,岳阳,414000;湖南省岳阳市中心血站检验科,414000; |
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| 摘 要： | Objective To prepare the polyclonal antibody against α1-antitrypsin(α1-AT)by immunizing rabbits with α1-AT,and to purify and characterize the antibodies produced from rabbits in order to establish the production process of the polyclonal antibody against α1-AT.Methods The healthy rabbits were routinely immunized with α1-AT to prepare polyclonal antibody against α1-AT.The serum antibody titers were determined by ELISA.The rabbits with qualified antibody titers were killed to collect blood.The antisera,obtained by centrifugalizing the whole blood,were purified by caprylic acid-ammonium sulfate precipitation and protein A affinity chromatography.The purified polyclonai antibodies were analyzed by 15%SDS-PAGE,and antibody titers weIe measured by ELISA.Results ELISA assay showed that the titers of antisera were over 105.The polyclonal antibodies,purified by caprylic acid-ammonium sulfate precipitation and protein A affinity chromatography,had good purity and immunological activity.Conclusion The production and purification processes of polyclonal antibody against α1-AT are successfully established.
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| 关 键 词： | α1抗胰蛋白酶 多克隆抗体 制备 纯化 鉴定 |
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