| 转化三七提取物为人参皂苷Rg3的真菌分离鉴定 |
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| 引用本文： | 王青,袁萍,茅仁刚,王菊勇,郑展.转化三七提取物为人参皂苷Rg3的真菌分离鉴定[J].华东师范大学学报(自然科学版),2011,2011(6):115-121,151. |
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| 作者姓名： | 王青 袁萍 茅仁刚 王菊勇 郑展 |
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| 作者单位： | 1. 上海中医药大学附属龙华医院中医肿瘤研究所,上海,200032
2. 上海师范大学生命与环境科学学院,上海,200234 |
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| 基金项目： | 上海市科委创新行动计划项目(09140903600);上海市教委项目(10YZ51);上海高校选拔培养优青项目(SZY10053);龙华医院自然科学研究基金(2011YM15) |
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| 摘 要： | 从培养人参(Panar ginseng C.A.Meyer)的土壤中筛选出23株菌,分别进行三七(Panax Notoginseng)提取物转化实验.经过摇瓶发酵后,采用TLC法与HPLC法对其发酵液提取物进行分析.研究结果表明,能够转化三七提取物为高活性人参皂苷的微生物菌株有9株,其中以菌株TR-20的转化作用最强....
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| 关 键 词： | 三七 人参皂苷Rg3 菌种筛选 菌种鉴定 |
| 收稿时间： | 2010-11-1 |
| 修稿时间： | 2011-2-1 |
Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg3 |
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| WANG Qing
,
YUAN Ping
,
MAO Ren-gang
,
WANG Ju-yong
,
ZHENG Zhan.Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg3[J].Journal of East China Normal University(Natural Science),2011,2011(6):115-121,151. |
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| Authors: | WANG Qing YUAN Ping MAO Ren-gang WANG Ju-yong ZHENG Zhan |
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| Affiliation: | 1(1.Research Institute of Cancer,Shanghai Academy of Traditional Chinese Medicine,Shanghai 200032,China; 2.School of Life Science,Shanghai Normal University,Shanghai 200234,China) |
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| Abstract: | Twenty-three strains were isolated from soil where ginseng grew. The Panax Notoginseng extract were analyzed by TLC and HPLC. There were nine strains which can converte Panax Notoginseng extract into highly active ginsenoside, among which the TR-20 was the best one. Moreover, the TR-20 can convert Panax Notoginseng extract into ginsenoside Rg3(190.2 mg/L). The TR-20 was Aspergillus (Aspergillus sp.) with observation of morphology and cultural characteristics. |
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| Keywords: | Panax Notoginseng|ginsenoside Rg3|strain screening|strain identification |
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