糖化酶活力测定方法研究

烟用酶制剂中糖化酶的活力是烟草工业生产中的重要指标。建立了3,5-二硝基水杨酸（DNS）测定糖化酶活力的方法。结果表明,在碱性条件下,糖化酶水解液与DNS反应产物的最大吸收波长为480 nm,线性范围为1.30～171.5 U/mL,加标回收率为99.8%～103.4%,RSD为0.7%～2.9%,检出限为1.58 U/mL,该方法可用于烟用酶制剂样品中糖化酶的测定,与标准方法相比,其准确性符合要求,且具有简便、快速的特点。

Study on the Determination of Glucoamylase Activity of Enzyme

Glucoamylase activity of enzyme used in tobacco is an important index to tobacco industrial manufacturation.A method to determine glucoamylase activity by spectrophotometry with 3,5-dinitrosalicylic acid had been developed.The results indicated that under alkaline conditions,the maximum absorption wavelength of 3-amino-5-nitro-salicylic acid,which was formed by the reaction of glucose and 3,5-dinitrosalicylic acid,was 460nm,the linear relationship was in the range of 1.30～171.5 U/mL,the recovery rate was 99.8 %～103.4 %,and RSD was 0.7 %～2.9 %,the detection limit was 1.58 U /mL.Such method could be applied in the determination of glucoamylase activity in enzyme samples for tobacco use.Compared with standard method,it had the advantages including high accuracy,simple and rapid operation.