| 猪伪狂犬病病毒TaqMan-MGB荧光定量PCR检测方法的建立及应用 |
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| 引用本文： | 刘园园,吴晖,肖性龙,贾赟,吴斌,余以刚.猪伪狂犬病病毒TaqMan-MGB荧光定量PCR检测方法的建立及应用[J].中国畜牧兽医,2009,36(4):76-79. |
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| 作者姓名： | 刘园园 吴晖 肖性龙 贾赟 吴斌 余以刚 |
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| 作者单位： | （1.华南理工大学轻工与食品学院，广州 510640； 2.深圳太太基因工程有限公司，深圳 518057；3.辽宁省检验检疫局技术中心，大连 116001） |
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| 摘 要： | 本研究针对猪伪狂犬病病毒的gE基因序列进行分析比较，设计了一条TaqMan-MGB探针，建立了区别野毒株及基因缺失疫苗株的荧光定量PCR检测方法。结果表明，该方法检测猪伪狂犬病病毒特异性强，能很好的区分猪伪狂犬病病毒与其它病毒。用该方法对83份疑似样本进行检测，与常规PCR检测法符合率达到100%，表明该方法用于检测猪伪狂犬病病毒具有良好的实用性。
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| 关 键 词： | 猪伪狂犬病病毒 TaqMan-MGB荧光定量PCR 检测 |
Establishment and Application of Fluorescence Quantitative TaqMan-MGB PCR Assay for Pseudorabies Virus |
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| LIU Yuan-yuan,WU Hui,XIAO Xing-long,JIA Yun,WU Bin,YU Yi-gang.Establishment and Application of Fluorescence Quantitative TaqMan-MGB PCR Assay for Pseudorabies Virus[J].China Animal Husbandry & Veterinary Medicine,2009,36(4):76-79. |
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| Authors: | LIU Yuan-yuan WU Hui XIAO Xing-long JIA Yun WU Bin YU Yi-gang |
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| Affiliation: | (1.College of Light Industry & Food, South China University of Technology, Guangzhou 510640,China; 2.Shenzhen Taitai Genomics, Inc., Shenzhen 518057,China;3. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China) |
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| Abstract: | A pair of specific primers and one TaqMan-MGB probe were designed based on gE gene through comparison and analysis,the FQ-PCR method was successfully constructed which could distinguish wild strain and gene-deleted vaccine strain.This method had the characteristic of high specificity to differentiate PRV form other virus.A comparison between FQ-PCR assay and conventional PCR assay were performed with 83 tissue samples,results showed that two methods match well.It indicates that TaqMan-MGB FQ-PCR assay provi... |
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| Keywords: | pseudorabies virus(PRV) fluorescence quantitative PCR（FQ-PCR） detect |
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