HPLC法测定桐叶中齐墩果酸和熊果酸的含量

目的：建立桐叶中齐墩果酸和熊果酸的含量测定方法。方法：采用HPLC法，色谱柱为PromosilC18柱（4．6minx250mm，5μm），流动相为乙睛-甲醇-水-磷酸（66：12：22：0．02），流速为1．0mL·main-1检测波长为210nm，柱温为25℃，进样量为20μL。结果：齐墩果酸在0．1292—1．5504μg范围内呈良好的线形关系（r=0．9999），平均回收率为100．1％，RSD％为3．4％（n=6）。熊果酸在O．2428—2．9136μg范围内呈良好的线形关系（r=O．9999），平均回收率为99-3％，RSD％）为2．2％（n=6）。结论：该方法操作简单、准确，重现性好，可用于桐叶的质量控制。

Determination of Oleanolic Acid and Ursolic Acid in Paulownia Leaves by HPLC

Objective ：To establish a method for the determination of oleanolic acid and ursolic acid in Paulownia Leaves. Methods：HPLC was applied, and column was Promosil C18（4.6 mm×250mm, 5μm）; mobile phase was acetonitrile- methanol - water-phosphoric acid （66：12：22：0.02）;flow rate was 1.0 mL＇min-1; the detection wavelength was 210 nm; column temperature was 25 ℃; the injection volume was 20 μL. Results：Oleanolic acid in the range of 0.1296-1.5504μg showed good linear relationship （r=0.9999）,and the average recovery was 100.1%; RSD% was 3.4% （n=6）. Ursolic acid in the range of 0.2428- 2.9136 μg showed good linear relationship （r=0.9999）, and the average recovery was 99.3%; RSD% was 2.2% （n=6）. Conclusion： The method is simple, accurate, reproducible,and can be used for Paulownia Leaves quality control.