| Effect of Electromagnetic Fields on Proliferation and Differentiation of Cultured Mouse Bone Marrow Mesenchymal Stem Cells |
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| 作者姓名： | 吴华 任凯 赵文春 葛保剑 彭松林 |
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| 作者单位： | Department of Orthopedics，Tongji Hospital，Tongji Medical College，Huazhong Universityof Science and Technology，Wuhan 430030，China，Department of Orthopedics，Tongji Hospital，Tongji Medical College，Huazhong Universityof Science and Technology，Wuhan 430030，China，Department of Orthopedics，Tongji Hospital，Tongji Medical College，Huazhong Universityof Science and Technology，Wuhan 430030，China，Department of Orthopedics，Tongji Hospital，Tongji Medical College，Huazhong Universityof Science and Technology，Wuhan 430030，China，Department of Orthopedics，Tongji Hospital，Tongji Medical College，Huazhong Universityof Science and Technology，Wuhan 430030，China |
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| 基金项目： | This project was supported by a grant from the National Natural Sciences Foundation of China (No. 50347025). |
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| 摘 要： | Previous clinical observations and animal ex periments showed that EMFs could stimulate newbone formation of the fractures and increase thebone mineral density1], but the manner in whichEMFs influence the behavior of bone remains poor ly understood. Recent studies2] found that signaltransduction system plays an important role in thebiological effect of EMFs. Intracellular cAMP wasan important second messenger in signal transduc tion pathway. The cellular proliferation …
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| 关 键 词： | 电磁场 细胞增殖 骨髓间质干细胞 小鼠 动物实验 |
| 收稿时间： | 10 June 2004 |
Effect of electromagnetic fields on proliferation and differentiation of cultured mouse bone marrow mesenchymal stem cells |
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| Wu Hua,Ren Kai,Zhao Wenchun,Ge Baojian,Peng Songlin.Effect of Electromagnetic Fields on Proliferation and Differentiation of Cultured Mouse Bone Marrow Mesenchymal Stem Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(2):185-187. |
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| Authors: | Wu Hua Ren Kai Zhao Wenchun Ge Baojian Peng Songlin |
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| Affiliation: | Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Summary: In order to study the effects of electromagnetic fields (EMFs) on proliferation, differentiation and intercellular cyclic AMP (cAMP) in mouse bone marrow mesenchymal stem cells (MSCs) in vitro, the mouse bone MSCs were isolated and cultured in vitro. The third passage MSCs were divided into 4 groups and stimulated with EMFs. The cellular proliferation (MTT), the cellular differentiation (alkaline phosphatase activity, ALP), and the intercellular cAMP level were investigated at different time points. The results showed that EMF (50Hz pulse burst 2 mT peak) inhibited the cellular proliferation (P<0.05), enhanced the cellular differentiation (P<0.05), and increased the intercellular cAMP level (P<0.01) in the early time of the stimulation (1-3 days), but the intercellular cAMP level did not increased further in the later days. We are led to conclude that the cAMP may be involved in the mediation of the growth inhibitory and differentiation-inducing signals of specific EMFs in vitro. |
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| Keywords: | electromagnetic field cyclic AMP alkaline phosphatase marrow mesenchymal stem cells |
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