| 结直肠癌组织中GITRL mRNA表达水平的检测及其临床意义 |
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| 引用本文： | 邹晨,陈锁成,吉均祥,蒋鹏程,王胜,陈建国,杨先知,王胜军.结直肠癌组织中GITRL mRNA表达水平的检测及其临床意义[J].江苏大学学报(医学版),2011,21(4):349-352. |
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| 作者姓名： | 邹晨 陈锁成 吉均祥 蒋鹏程 王胜 陈建国 杨先知 王胜军 |
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| 作者单位： | 1. 江苏大学附属人民医院普外科,江苏,镇江,212002
2. 江苏大学基础医学与医学技术学院免疫学系,江苏,镇江,212013 |
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| 基金项目： | 国家自然科学基金资助项目，江苏省卫生厅面上项目，江苏省自然科学基金资助项目，镇江市社会发展基金资助项目 |
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| 摘 要： | 目的:建立检测人糖皮质激素诱导的肿瘤坏死因子受体配体(human glucocorticoid induced tumor necrosis factor receptor ligand,hGITRL)mRNA的实时荧光PCR方法,探讨hGITRL表达与结直肠癌发生发展的关系.方法:构建含hGITRL基因片段的标准质粒...
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| 关 键 词： | 结直肠癌 糖皮质激素诱导的肿瘤坏死因子受体配体 实时荧光定量PCR |
Detection and significance of human GITRL mRNA in colorectal cancer tissues |
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| ZOU Cheng,CHEN Suo-cheng,JI Jun-xiang,JIANG Peng-cheng,WANG Sheng,CHEN Jian-guo,YANG Xian-zhi,WANG Sheng-jun.Detection and significance of human GITRL mRNA in colorectal cancer tissues[J].Journal of Jiangsu University Medicine Edition,2011,21(4):349-352. |
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| Authors: | ZOU Cheng CHEN Suo-cheng JI Jun-xiang JIANG Peng-cheng WANG Sheng CHEN Jian-guo YANG Xian-zhi WANG Sheng-jun |
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| Affiliation: | 1.Department of General Surgery,the Affiliated People′s Hospital of Jiangsu University,Zhenjiang Jiangsu 212002;2.Department of Immunology,School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
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| Abstract: | Objective: To establish a method of real-time fluorescence PCR for human glucocorticoid induced tumor necrosis factor receptor ligand(hGITRL) mRNA and investigate the relationship between the expression of hGITRL gene and colorectal cancer occurrence.Methods: The standard plasmid of hGITRL gene was firstly constructed.The levels of expression of GITRL in colorectal cancer tissues(n=20) were detected by real time PCR.The correlation between GITRL and colorectal cancer occurrence were analysed.Results: The re... |
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| Keywords: | colorectal cancer human glucocorticoid induced tumor necrosis factor receptor ligand real time PCR |
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