日本樱花茎段再生体系的建立

以成年日本樱花茎段为外植体,系统研究了取材时间、冷藏时间、消毒方法、激素等诸多因素对茎段再生的影响,建立了日本樱花茎段丛生芽再生体系。研究结果表明,一般中午取材、4℃冷藏2 d、75%酒精浸泡3 min后用0.1%HgCl2浸泡7 min,可获得日本樱花无菌材料。将无菌材料接种至MS+NAA0.05 mg.L-1+6-BA2.0 mg.L-1的分化培养基上,腋芽明显萌动并伸长;在MS+6-BA4.0 mg.L-1+NAA0.3 mg.L-1+GA30.05 mg.L-1的增殖培养基上,腋芽不但能较快增殖形成大量丛生芽,而且形成的小芽能继续长大。待小芽长至3 cm~3.5 cm时,将其切割下来转移至1/2MS+NAA0.6 mg.L-1+IBA0.2 mg.L-1生根培养基中,最终获得日本樱花的完整植株。

Establishment of the Regeneration System of Stem Segments of Prunus yedoensis

Prunus yedoensis is one of famous ornamental trees,which plays a very important role in city afforestation.In this paper,a discussion was made on the P.yedoensis stem segment regeneration system.The shoots of the second time extraction were used as the experimental material.Researches were conducted on several factors such as sampling time,cold storage time,sterilization methods,hormone concentration,and so on to optimize the regeneration system in vitro.The experimental results showed that the pretreatment method to the explant could greatly affect P.yedoensis regeneration.The best method was to do sampling in the noon and to carry out cold storage for 2 days at 4℃.It showed that the treatment of 75% ethanol for 3 minutes ＋ 0.1% HgCl2 for 7 minutes was no good to the explants.The high frequency of axillary buds regeneration was observed when stem segment explants were cultured on MS medium supplemented with 2.0 mg·L-1 6-BA and 0.05 mg·L-1 NAA.The high proliferation times and elongation of adventitious buds were observed when axillary buds were cultured on MS medium supplemented with 4.0 mg·L-1 6-BA,0.3 mg·L-1 NAA and 0.05 mg·L-1 GA3.The buds were transferred into the rooting medium when it reached to 3 cm~3.5 cm.The best rooting medium for the radication was 1/2MS medium supplemented with 0.6 mg·L-1 NAA and 0.2 mg·L-1 IBA and developed into whole plantlets.