利用全基因组寡核苷酸芯片初步筛选无功能垂体腺瘤相关基因

目的探讨人无功能垂体腺瘤发生、发展中相关基因的表达与功能。方法应用人全基因组寡核苷酸芯片HG-U133Plus2.0分别检测4例无功能垂体腺瘤组织(2例促性腺激素腺瘤、2例裸细胞激素腺瘤)和正常垂体组织的基因表达谱,并对差异表达基因进行筛选和生物信息学分析。同时随机选择EDG3基因予以实时荧光定量PCR验证。结果与正常垂体组织相比,促性腺激素腺瘤中上调基因223条,下调基因678条;而裸细胞腺瘤中上调基因156条,下调基因665条;两者共同表达基因中上调基因50条,下调基因136条。共同差异表达基因的功能主要涉及分子结合、凋亡或肿瘤相关、代谢、信号传导、细胞周期、物质运输等多个生物过程。结论基因芯片技术有助于在分子水平上了解垂体腺瘤发病机制。

Preliminary screening for associated genes in nonfunctional pituitary adenoma by whole genome oligonucleotide microarrays

Objective To investigate the expression and function of associated genes in development and progress of human nonfunctional pituitary adenoma（NFPA）.Methods Gene expression profiling in 4 NFPA samples（2 gonadotroph cell adenomas and 2 null cell adenomas）and normal pituitary tissues was examined by the whole genome oligonucleotide microarray-Affymetrix 133 Plus 2.0,respectively.Then the differentially expressed genes were screened and analyzed by bioinformatics,and a gene EDG3 was randomly selected to verify the microarray result by real-time fluorescence quantitative PCR.Results Compared with normal pituitary tissues,223 genes were up-regulated and 678 genes were down-regulated in gonadotroph cell adenomas,however,156 genes were up-regulated and 665 genes were down-regulated in null cell adenomas.Fifty up-regulated and 136 down-regulated genes were co-expressed in both gonadotroph cell and null cell adenomas.These differentiated genes were associated with many biological processes such as molecular association,apoptosis or related to tumor,metabolism,signal transduction,cell cycle,material transportation.Conclusions The microarray technology is helpful to understand the pathogenesis of NFPA at a molecular level.