| 高纯度表没食子儿茶素没食子酸酯（EGCG）的分离与制备 |
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| 引用本文： | 戚向阳 谢笔钧. 高纯度表没食子儿茶素没食子酸酯（EGCG）的分离与制备[J]. 精细化工, 1994, 11(4): 40-46 |
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| 作者姓名： | 戚向阳 谢笔钧 |
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| 作者单位： | 华中农业大学食品科学系 |
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| 基金项目： | 农业部“八五”重点课题 |
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| 摘 要： | 绿茶的乙醇浸提液经过浓缩、脱色和萃取，得到茶多酚粗品。用葡聚糖凝胶ＬＨ－２０层析柱和制备型ＨＰＬＣ分离提纯儿茶素组分ＥＧＣＧ，结构鉴定结果证明所获得的ＥＧＣＧ其纯品达到色谱纯级。
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| 关 键 词： | 茶叶 分离 茶素 茶多酚 |
ISOLATION AND PREPARATION OF HIGH PURITY EPIGALLOCATECHIN GALLATE(EGCG) |
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| Abstract: | Abstract In this paper,we reported a method forisolation and purification of catechins from teapolyphenol in green tea.The polyphenol was obtained from greentea by soaking it with 80%ethyl alcohol,thenusing No. 2 solvent for decolorization and de-caffeination and extracting catechins in theaqueous layer with EtAc.The content of cate-chins was 75.43%, the product percent was12.53%.Catechins was separated by automaticcolumn chromatography using a columnpacked with Sephadex LH-20,then furtherseparated and purified by preparative HPLC.Responding to the first peak of preparativeHPLC,it was chromatographically pureEGCG,identified by UV,IR,HPLC,￣1HNMRandJement analysis. |
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