| 产脂肪酶假丝酵母的筛选及其研究 |
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| 引用本文： | 金波,罗杰,余斐,Mei Xingguo,梅兴国.产脂肪酶假丝酵母的筛选及其研究[J].华中科技大学学报(自然科学版),2001,29(5):111-113. |
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| 作者姓名： | 金波 罗杰 余斐 Mei Xingguo 梅兴国 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院
2. Postgraduate; College of Life Sci. & Tech., HUST, Wuhan 430074, China |
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| 摘 要： | 从武汉油污样中分离获得一株高产脂肪酶酶的假丝酵母R－2．发酵研究表明，最适培养基为蛋白胨20g/L,蔗糖10g/L,橄榄油5g/L,(NH4)2SO41g/L,MgSO4.7H2O1g/L,K2HPOR1g/L和吐温－801g/L,最适产酶条件为30℃，初始pH7．0,转速200r/min，培养32h ,最高产酶活力达到1437u/mL，酶作用最适温度40℃，最适pH7.5。
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| 关 键 词： | 脂肪酶 假丝酵母 产酶条件 培养基 产酶活力 蛋白胨 蔗糖 |
| 文章编号： | 1000-8616(2001)05-0111-03 |
| 修稿时间： | 2000年10月30 |
Screening of Lipase Producing Strain of Candida sp and the Characteristic of Its Lipase |
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| Mei Xingguo.Screening of Lipase Producing Strain of Candida sp and the Characteristic of Its Lipase[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2001,29(5):111-113. |
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| Authors: | Mei Xingguo |
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| Abstract: | A lipase-producing strain Candida R-2 is isolated from oil ontained earth samples. The optimal medium for lipase production has been found: peptone 20 g/L, sucrose 10 g/L, olive oil 5 g/L, (NH4)2SO4 1 g/L, MgSO4 ·7H2O 1 g/L, K2HPO4 1 g/L and tween-80 1 g/L. And the optimal culture condition is cultured at initial pH7.0 at 30℃ for 32 hours with a rotating speed of 200 r/min. The maximum lipase production reaches 1 437 u/mL, and the optimal pH and temperature for the lipase are 7.5 and 40℃, respectively. |
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| Keywords: | lipase Candida sp condition of enzyme production |
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