GAO Feng~1,LI Wei~2,TANG Zon

tific Experiment Center of Guangxi Medical University,Nanning 530021,China)Objective:To investigate the mutation pattern of adenomatous polyposis coli(APC),Kirsten-ras(K-ras) and p53 genes in sporadic colorectal cancer tissues.Meth

ods:DNA was extracted by phenol-chloroform method from tumor and normal

ed using polymerase chain reaction(PCR),single-strand conformation polymorphism(SSCP) analysis,and DNA sequencing methods.Results:The mutations of APC,K-ras,and p53 was 37.5%(18/48),43.8%(21/48),and 35.4%(17/48),respectively.Forty two of 48 patients(87.5%) contained mutations in at least one of these genes including one gene mutation,two or three gene mutations.The rate of only one gene mutation of APC,K-ras,and p53 was 16.7%(8/48),25.0%(12/48),and 20.8%(10/48),respectively.The total rate of one gene mutation was 62.5%(30/48).The mutations in both APC and p53 was 6.25%(3/48),in both APC and K-ras was 10.42%(5/48),in both p53 and K-ra