后半规管阻塞术对内耳功能与形态影响的实验观察

本实验对１５只豚鼠行后半规管阻塞术，术前、术后测定ＣＡＰ阈值和ＥＮＧ，并作常规火棉胶连续切片及透射电镜观察内耳形态变化。结果显示：ＣＡＰ阈值手术前、后无显著性差异（Ｐ＞０．０５）；眼震持续时间，术后１周明显缩短（Ｐ＜０．０１），术后８周接近术前（Ｐ＞０．０５）。术后１周，光镜下见耳蜗底圈前庭膜向蜗管内膨隆，其余结构无明显异常；电镜观察见椭圆囊、壶腹峙毛细胞个别线粒体肿胀，耳蜗毛细胞正常。术后８周组内耳各结构正常。初步表明该手术对内耳功能与结构无永性损害。

Changes of Function and Morphology of the Inner Ear in Guinea Pigs with Posterior Semicircular Canal Occlusion

Posterior semicircular canal occltlsion in 15 guinea pig,was performed.Auditory(CAP)and vestibular function were measured before and after surgical occlusion. Morphological changes of the inner ear were observed using light micrascopy and transmission electron mi-crcopy. The results showed that there was no significant change in threshold of CAP before and after surgery,and that the duration of nystagmus was significantly decreased in the first week after operation,with no significant difference in the eighth week.The light microscopy investigation of the inner ear showed a normal structure except vestibular menbrane of basal tunr of the cochlea in the first week after canal occlusion. Finding in transmission electron miczrrscopy was edema of a few mitochondria in the hair cells of the utricle and crista ampulla in the eighth week.Ultrastruc-ture of hair cells of cochlea had showed no change. The results indicate that pcsterior semicircular canal occlusion may not pern1anently do damage to the function and morphoIogy of the inner ear.