Maspin、bFGF在宫颈病变中的表达及与高危型HPV的相关性研究

目的:通过检测乳腺丝氨酸蛋白酶抑制剂(Maspin)、碱性成纤维细胞生长因子(bFGF)在慢性宫颈炎、宫颈上皮内瘤样变(CIN)及宫颈癌组织中的表达,探讨两者的相关性及与高危型人乳头状瘤病毒(高危型HPV)感染之间的关系。方法:宫颈石蜡组织标本166例,其中高危型HPV(+)组106例(慢性宫颈炎20例、CINⅠ级20例、CINⅡ级20例、CINⅢ级20例、宫颈癌组26例);HPV(-)组60例(CINⅠ级35例、CINⅡ级20例、CINⅢ级5例)。应用SP法检测Maspin、bFGF的表达情况。结果:(1)在高危型HPV(+)组中,Maspin蛋白的阳性表达率呈逐渐下降趋势(P<0.05),且在CINⅢ级组中的表达同慢性宫颈炎组、CINⅠ级组、CINⅡ级组相比,差异均有统计学意义(均P<0.05),在宫颈癌组中的阳性表达与CIN各组、慢性宫颈炎组相比较,差异均有统计学意义(均P<0.05);bFGF的阳性表达率呈逐渐增加趋势(P<0.05),bFGF阳性表达率在宫颈癌组表达率最高,显著高于慢性宫颈炎组、CINⅠ级组、CINⅡ级组,差异均有统计学意义(均P<0.05)。在HPV(-)组中,Maspin蛋白的表达依次降低,各组间比较差异均无统计学意义(P>0.05);bFGF的表达依次升高,但差异无统计学意义(P>0.05)。(2)Maspin、bFGF蛋白的表达呈负相关,两者与高危型HPV分别呈负、正相关。结论:高危型HPV可能通过P53调节通路下调Maspin的蛋白表达及上调bFGF的蛋白表达,导致CIN直至宫颈癌的发生。

Expression of Maspin, bFGF in the cervix lesions and correlation with high-risk type HPV

Objective: To detect the expression of mammary serpin(Maspin),basic fibroblast growth factor(bFGF) in patients with chronic cervicitis,cervical intraepithelial neoplasia and cervical cancer and explore the relationship between Maspin,bFGF and high-risk human papilloma virus(high-risk type HPV) infection.Methods: Cervical paraffin tissue samples were collected(n=166) in which 106 cases were in high-risk type HPV positive group(chronic cervicitis 20 cases,CINⅠgrade 20 cases,CINⅡ grade 20 cases,CINⅢgrade 20 cases,cervical cancer 26 cases);60 cases were in HPV negative group(CINⅠgrade 35 cases,CINⅡgrade 20 cases,CINⅢ grade 5 cases).Expression of Maspin,bFGF protein was detected by Immunohistochemical SP method.Results:(1)High-risk type HPV(+) group: The rate of Maspin protein positive expression was gradually decreased(P <0.05),The level of Maspin protein positive expression in CIN Ⅲ was statistically lower than those of chronic cervicitis,CINⅠgrade group,and CIN Ⅱ grade group(P <0.05),The rate of Maspin protein positive expression in cervical cancer was significantlly lower than those in each group of CIN,chronic cervicitis group(P <0.05);The positive expression rate of bFGF was significantly increasing(P <0.05),The positive rate of bFGF expression in the cervical cancer was highest,It was significantly higher than the rate of chronic cervicitis,CINⅠgrade group,CINⅡgrade group(P <0.05).HPV(-) group of CIN :The positive rates of Maspin protein were gradually lower among CIN groups but the difference between groups was not statistically signif icant(P>0.05);The positive rate of bFGF in CIN groups was gradually increased but the difference was not statistically significant(P>0.05).(2)The relationship of Maspin protein expression and bFGF protein expression was negatively correlated.the relationship of Maspin protein expression and high-risk type HPV infection was negatively correlated and the relationship of bFGF protein expression and high-risk type HPV infection was positively correlated,by using Spearman correlation analysis.Conclusion: High-risk type HPV may degrade Maspin protein expression and upgrade bFGF protein expression through P53 way,and result in the cervical leision from chronic cervicitis to CIN to cervical cancer.