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西藏产双孢菇(Agaricus bisporus)挥发性呈香成分初探
引用本文:闫素君,谢惜媚,林素贞,徐爱国,蒋思萍,陈彬,刘在民,刘岚.西藏产双孢菇(Agaricus bisporus)挥发性呈香成分初探[J].中山大学学报(自然科学版),2015,54(1).
作者姓名:闫素君  谢惜媚  林素贞  徐爱国  蒋思萍  陈彬  刘在民  刘岚
作者单位:1. 中山大学化学与化学工程学院,广东 广州 510275;
2. 中山大学测试中心,广东 广州 510275; 
3. 西藏自治区高原生物研究所高原真菌重点实验室,西藏自治区 拉萨 850001;
4. 中山大学海洋学院,广东 广州 510275
基金项目:国家重点基础研究发展计划(973计划)资助项目(2012CB126308);西藏自治区重点科技项目资助项目《毛瓣绿绒蒿等濒危藏药材内生真菌次级代谢产物及其与宿互惠共存关系研究》
摘    要:文章采用水蒸气蒸馏法提取来自西藏的3个双孢菇样品(Agaricus bisporus)的挥发油,以1个广州样品作为对照。通过加入薄荷醇作为参照标准物质的方法,应用气相色谱-质谱法结合NIST谱库检索研究了样品中的挥发性化学成分和相对含量,从4个双孢菇样品中分析鉴定了40个化合物。藏产编号为2796,709,694中的含量分别为43.10%,37.15%,31.34%,其中含量较高的挥发性成分依次为苯甲醇、糠醛、苯甲醛和柏木醇;广州样品0107号的含量为27.24%,其中除含量较高的苯甲醇、糠醛、糠醇外,尚有2-(1,1-二甲基乙基)-3-甲基-环氧乙烷,2-丙烯醇和马索亚内酯。研究结果表明菌种来源、产地气候、栽培方式会影响双孢菇的风味,为双孢菇的综合开发利用提供了理论依据。

关 键 词:双胞菇  挥发性化学成分  水蒸气蒸馏  气相色谱  -质谱联用

Study on Volatile Flavor Components from Agaricus bisporus of Tibet
YAN Sujun,XIE Ximei,LIN Suzhen,XU Aiguo,JIANG Siping,CHEN Bin,LIU Zaimin,LIU Lan.Study on Volatile Flavor Components from Agaricus bisporus of Tibet[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2015,54(1).
Authors:YAN Sujun  XIE Ximei  LIN Suzhen  XU Aiguo  JIANG Siping  CHEN Bin  LIU Zaimin  LIU Lan
Affiliation:1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China;
2. Instrumental and Analysis Research Center, Sun Yat-sen University, Guangzhou 510275, China;
3. Key Laboratory of Plateau Fungus, Tibet Plateau Institute of Biology, Lhasa 850001, China;
4. School of Marine Sciences, Sun Yat sen University, Guangzhou 510275, China
Abstract:The volatile oil was extracted from 3 samples Agaricus bisporus of Tibet using a steam distillation method, compared with 1 sample of Guangzhou. Forty volatile compounds were identified with GC-MS analysis and the NIST spectral library search. Through an area normalization method by adding menthol as reference material, the relative contents of No.2796,No.709,No.694 from Tibet were determined to be 43.10%,37.15%,31.34% respectively with the high content volatile compounds benzyl alcohol, furancarboxaldehyde, benzaldehyde, Cedrol. While No.0107 from Guangzhou was 27.24% with compounds benzyl alcohol, furancarboxaldehyde, benzaldehyde, 2-(1,1-dimethylethyl)-3-methyl-Oxirane, 5,6-dihydro-6-pentyl-2H-Pyran-2-one and 2-Propen-1-ol. The results showed that fungus source, origin of climate, cultivation methods affected the flavor of Agaricus bisporus and provided theoretical basis for the multiple development and utilization of Agaricus bisporus.
Keywords:Agaricus bisporus  volatile component  steam distillation  GC-MS
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