| 丝氨酸蛋白酶Omi/HtrA2在肝癌细胞系中的表达及其促凋亡作用 |
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| 引用本文： | 徐宗全,刘恩宇,彭程,牛卫博,李允光,徐克森,牛军.丝氨酸蛋白酶Omi/HtrA2在肝癌细胞系中的表达及其促凋亡作用[J].中国现代普通外科进展,2011,14(8):589-593. |
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| 作者姓名： | 徐宗全 刘恩宇 彭程 牛卫博 李允光 徐克森 牛军 |
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| 作者单位： | 山东大学齐鲁医院肝胆外科,山东 济南,250012 |
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| 基金项目： | 国家自然科学基金(30872460); 教育部博士点基金(20060422048) |
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| 摘 要： | 目的：探讨Omi/HtrA2丝氨酸蛋白酶活性对肝癌细胞HepG2凋亡的影响。方法：检测O-mi/HtrA2在正常肝上皮细胞L02细胞系和肝癌细胞系HepG2、Hep3B、PLC中的表达。用脂质体法将Omi/HtrA2小干扰RNA表达载体（psiRNA-Omi/HtrA2）转染至人HepG2细胞中,以RT-PCR和Western Blot法评价psiRNA-Omi/HtrA2对Omi/HtrA2表达的沉默效应。用MTT法和流式细胞仪检测siRNA导致Omi/HtrA2基因沉默后HepG2细胞凋亡的变化。结果：在L02细胞系和3种肝癌细胞系中,所有细胞系在mRNA水平和蛋白质水平均表达Omi/HtrA2,在肝癌细胞系中的Omi/HtrA2 mRNA和蛋白质表达水平均高于正常肝上皮细胞L02。psiRNA-Omi/HtrA2载体可特异性抑制HepG2细胞中Omi/HtrA2的表达。转染psiRNA-Omi/HtrA2载体的HepG2细胞凋亡下调。结论：Omi/HtrA2在肝癌细胞系中表达增高,它可能在促进肝癌细胞凋亡中有重要作用,Omi/HtrA2为肝癌的治疗提供了一个新的选择靶点。
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| 关 键 词： | Omi/HtrA2 L02 肝癌细胞系 凋亡 |
Experimental study on the expression of Omi/HtrA2 and its proapoptosis in hepatocelluar carcinoma cell lines |
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| XUZong-quan,LIU En-yu,PENG Cheng,NIU Wei-bo,LI Yun-guang,XU Ke-sen,NIU Jun.Experimental study on the expression of Omi/HtrA2 and its proapoptosis in hepatocelluar carcinoma cell lines[J].Chinese Journal of Current Advances in General Surgery,2011,14(8):589-593. |
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| Authors: | XUZong-quan LIU En-yu PENG Cheng NIU Wei-bo LI Yun-guang XU Ke-sen NIU Jun |
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| Affiliation: | XU Zong-quan,LIU En-yu,PENG Cheng,NIU Wei-bo,LI Yun-guang,XU Ke-sen,NIU Jun Department of Hepatobiliary Surgery.Qilu Hospital of Shandong University(Jinan 250012,China) |
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| Abstract: | Objective: To study the expression of the serine protease Omi/HtrA2 on apoptosis of hepatoma cells.Methods: Immunohistochemical technique,real time PCR and Westblot were used to investigate the expression of Omi/HtrA2 in normal hepatocelluar cell line L02,and hepatocelluar carcinoma cell lines;The psiRNA-Omi/HtrA2 was transiently transfected into HepG2 cells and the inhibited effect of psiRNA-Omi/HtrA2 on expression of Omi/HtrA2 was detected using RT-PCR and Western Blot;the apoptosis of cancer cells was de... |
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| Keywords: | Omi/HtrA2·Cell L02·Hepatocelluar carcinoma cell lines·Apoptosis |
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