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| BDNF基因修饰淋巴细胞培养上清对 PC12细胞氧化应激损伤的保护作用 | |
| 引用本文： | 黄居科,唐小卿,潘渊明,冯鉴强,陈培熹,陈明振.BDNF基因修饰淋巴细胞培养上清对 PC12细胞氧化应激损伤的保护作用[J].中山大学学报(医学科学版),2004,25(6):524-529. |
| 作者姓名： | 黄居科 唐小卿 潘渊明 冯鉴强 陈培熹 陈明振 |
| 作者单位： | 1. 深圳市宝安区人民医院神经外科,广东,深圳,518000;中山大学附属第一医院神经外科 2. 中山大学基础医学院生理学教研室 3. 中山医学院麻醉学系,广东,广州,510080 4. 中山大学附属第一医院神经外科 |
| 摘 要： | 目的] 研究脑源性神经营养因子基因( brain- derived neurotrophic factor, BDNF gene)修饰淋巴细胞对 PC12细胞氧化应激损伤的保护作用.方法] MTT法检测 H2O2和多巴胺( dopamine, DA)对 PC12细胞生长的抑制率, Hoechst染色和 PI染色流式细胞仪( flow cytometry, FCM)检测 H2O2和 DA对 PC12细胞凋亡的诱导作用.结果] BDNF基因修饰的大鼠淋巴细胞的培养上清可降低 H2O2和 DA对 PC12细胞生长的抑制率并能抑制 H2O2和 DA诱导 PC12细胞凋亡.结论] BDNF基因修饰淋巴细胞的培养上清对 PC12细胞氧化应激损伤具有保护作用. |
| 关 键 词： | 脑源性神经营养因子 淋巴细胞 氧化应激 PC12细胞 |
| 文章编号： | 1672-3554(2004)06-0524-06 |
| 修稿时间： | 2004年6月20日 |
Protective Effect of Supernatant of Lymphocytes Modified by Brain- derived Neurotrophic Factor Gene on PC12 Cells Damage Induced by Oxidative Stress |
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| HUANG Ju-ke ,TANG Xiao-qing ,PAN Yuan-ming ,FENG Jian-qiang ,CHEN Pei-xi ,CHEN Ming-zhen.Protective Effect of Supernatant of Lymphocytes Modified by Brain- derived Neurotrophic Factor Gene on PC12 Cells Damage Induced by Oxidative Stress[J].Journal of Sun Yatsen University(Medical Sciences),2004,25(6):524-529. | |
| Authors: | HUANG Ju-ke TANG Xiao-qing PAN Yuan-ming FENG Jian-qiang CHEN Pei-xi CHEN Ming-zhen |
| Affiliation: | HUANG Ju-ke 1,3,TANG Xiao-qing 2,PAN Yuan-ming 4,FENG Jian-qiang 2,CHEN Pei-xi 2,CHEN Ming-zhen 3 |
| Abstract: | ObjectiveTo study the protective effect of lymphocytes modified by brain-derived neurotrophic factor gene (BDNF gene) on PC12 cells damage induced by oxidative stress. MethodsThe growth inhibitory rate of PC12 cells mediated by H2O2 and dopamine (DA) was evaluated by MTT assay. The apoptosis of PC12 cells induced by H2O2 and DA was determined by PI stain flow cytometry and Hoechst stain. ResultsThe supernatant of lymphocytes modified by BDNF gene decreased the growth inhibitory rate and apoptosis rate of PC12 cells induced by H2O2 and DA. ConclusionThe supernatant of rat lymphocytes genetically modified by BDNF gene has the protective effect on PC12 cells damage induced by oxidative stress. |
| Keywords: | brain-derived neurotrophic factor lymphocytes oxidative stress PC12 cells |
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