| DO11.10转基因小鼠脊髓损伤后的运动功能恢复 |
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| 引用本文： | 王慧,郭俊,赵宇,卞干兰,刘芳芳,于才勇,冯睿,鞠躬,王键.DO11.10转基因小鼠脊髓损伤后的运动功能恢复[J].细胞与分子免疫学杂志,2010,26(3). |
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| 作者姓名： | 王慧 郭俊 赵宇 卞干兰 刘芳芳 于才勇 冯睿 鞠躬 王键 |
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| 作者单位： | 1. 第四军医大学,基础部神经生物学教研室,陕西,西安,710032
2. 第四军医大学,唐都医院神经内科,陕西,西安,710038 |
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| 基金项目： | 军队"十一五"专项，军事医学重大项目 |
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| 摘 要： | 目的:通过比较两种小鼠-DO11.10和BALB/c小鼠脊髓损伤后不同的运动功能恢复程度和损伤区的形态学变化来分析T淋巴细胞对脊髓损伤修复的影响。方法:建立小鼠脊髓重度夹伤模型,HE染色,GFAP、CD11b和淋巴细胞免疫组织化学实验分析损伤14 d、21 d后损伤区的变化;在损伤后0 d、7 d、14 d和21 d进行Basso-Beattie-Bresnahan(BBB)运动评分来评价小鼠运动功能恢复情况。结果:脊髓损伤后21 d,BALB/c小鼠产生了较厚的胶质瘢痕并伴有明显的固缩现象;而DO11.10小鼠中只观察到稀薄的胶质瘢痕形成并没出现明显的固缩。DO11.10小鼠脊髓损伤后14 d,巨噬/小胶质细胞浸润较BALB/c小鼠明显增加。脊髓损伤后21 d,DO11.10小鼠脊髓损伤区中的T淋巴细胞较BALB/c小鼠明显减少。BBB运动评分显示,在损伤后21 d内DO11.10小鼠较BALB/c小鼠有显著的运动功能恢复。结论:脊髓损伤后21 d内,针对神经组织抗原的自身反应性T淋巴细胞的浸润和存在不利于脊髓损伤后的运动功能恢复。
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| 关 键 词： | 脊髓损伤 BBB评分 炎症反应 T淋巴细胞缺陷 |
Recovery of movement after spinal cord injury in DO11.10 transgenic mouse |
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| WANG Hui,GUO Jun,ZHAO Yu,BIAN Can-lan,LIU Fang-fang,YU Cai-yong,FENG Rui,JU Gong,WANG Jian.Recovery of movement after spinal cord injury in DO11.10 transgenic mouse[J].Journal of Cellular and Molecular Immunology,2010,26(3). |
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| Authors: | WANG Hui GUO Jun ZHAO Yu BIAN Can-lan LIU Fang-fang YU Cai-yong FENG Rui JU Gong WANG Jian |
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| Affiliation: | WANG Hui1,GUO Jun2,ZHAO Yu1,BIAN Gan-lan1,LIU Fang-fang1,YU Cai-yong1,FENG Rui1,JU Gong1,WANG Jian11Department of Neurobiology,School of Basic Medicine,Xi\'an 710032,2Department of Neurology,Tangdu Hospital,Fourth Military Medical University,Xi\'an 710038,China |
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| Abstract: | AIM:To analysis the role of T lymphocytes in spinal cord regeneration by comparing the recovery of movement and the morphological changes of injury area between BALB/c and DO11.10 transgenic mice.METHODS:Producing a crush injury model of spinal cord with special forceps.Analyze the changes of spinal cord injury area with H&E and GFAP,CD11b and lymphocytic immunohistochemical staining.Evaluate the recovery of movement function with Basso-Beattie-Bresnahan(BBB)locomotion testing system at 0,7,14 and 21 day po... |
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| Keywords: | spinal cord injury BBB locomotion testing inflammatory response T-lymphocyte defects |
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