膀胱移行细胞癌中Survivin与Ki-67、p53的表达及临床意义

目的探讨膀胱移行细胞癌中Survivin、p53及Ki-67的表达与膀胱移行细胞癌临床病理特征的关系及其临床意义。方法用免疫组织化学方法检测88例膀胱移行细胞癌与10例正常膀胱黏膜组织中Survivin、p53及Ki-67的表达，并用RT-PCR方法验证其中30例膀胱移行细胞癌和10例正常膀胱黏膜组织中Survivin的表达。结果免疫组织化学方法显示在膀胱移行细胞癌中Survivin、p53阳性表达率分别为63.6％（56／88）和45.5％（40／88），正常膀胱黏膜组织中均无表达，差异有统计学意义（P〈0.01），且Survivin的表达与膀胱癌的临床分期密切相关（P〈0.05）。膀胱癌组织中Ki-67增殖指数（PI）为20.4±10.7，正常膀胱黏膜组织中为0。RT-PCR方法显示在30例膀胱移行细胞癌中Survivin阳性表达率为100％，而正常膀胱黏膜组织中均无表达。结论Survivin与膀胱癌的恶性程度有关，与p53及Ki-67共同参与了膀胱移行细胞癌的发生、发展及进程。

The expressions of Survivin,p53 and Ki-67 in bladder cancer

Objective To study the expressions of Survivin, p53 and Ki-67 in bladder cancer tissue,and to explore their relationship with tumor prognosis. Methods The protein expression of Survivin,p53 and Ki-67 on 88 bladder cancer specimens and 10 normal tissue specimens （control） were tested immunohistochemically, and 30 of cancer specimens and all of the control were also tested by RT-PCR for the expression of their mRNA. Results The expression rates of Survivin and p53 in bladder cancer were 63.6%（56/88） and 45.5%（40/88）,whereas both were 0%（0/8） in the control（all P〈0.01）. Ki-67 proliferating index（PIin cancer tissue was 20.4±10.7, whereas that in normal tissue was 0. The result from RT-PCR indicated that Survivin mRNA was positive on all of 30 cancer tissue,and negative on all the normal bladder tissue. Conclusions The Survivin was associated with malignancy of bladder cancer, and together with p53 and Ki-67, contributed to the onset and development of bladder cancer.