从生猪中分离小肠结肠炎耶尔森菌病原学研究

目的 了解河南省登封市生猪中小肠结肠炎耶尔森菌的病原学特征。方法 以采集的1346份猪咽拭子所分离的331株小肠结肠炎耶尔森菌为研究对象，进行系统生化鉴定、血清学分型和毒力基因PCR检测。结果 采集的1346份猪咽拭子中共筛查、分离出小肠结肠炎耶尔森菌331株，分离率为24.59℅（331/1346）；鉴定出O:3、O:5、O:8三种血清型（84.6% ,2.1% 和1.8%）和1A、3、4三个生物型；毒力基因分布特征为ail+、ystA+、ystB-、yadA+、virF+占80.67℅（267/331），ail+、ystA+、ystB-、yadA-、virF-占3.32℅（11/331）且均为血清型O:3；其它型的为16.01℅（53/331）。结论 生猪为携带小肠结肠炎耶尔森菌的主要宿主，且多携带致病性菌株，提示在今后疫情控制中应加强生猪的监测、检疫和管理。

Study on the etiologic characteristics of Yersinia enterocolitica separated from Swine

【Abstract】　To investigate the pathogenic characteristics of Yersinia enterocolitica isolated from oral-pharyngeal swab (tonsils) samples in Dengfeng area. 331 strains of Y. enterocolitica were isolated from 1346 pharyngeal swab, the detection rate was 24.59%.These bacteria were analysed by the systematic biochemistry, serotyp and PCR detection methods. It were identified three serotypes(O:3,O:5,O:8)，of which 84.6% ,2.1% and 1.8% respectively.while they belonged to three biological type(1A, 3 and 4 ). Distribution characteristics of toxicity gene were as follow: ail+、ystA+、ystB-、yadA+、virF+ accounted for 80.67℅, ail+、ystA+、ystB-、yadA-、virF- accounted for 3.32℅,they were all O:3 serotype;others accounted for 16.01℅.From these observations,it is evident that swine is the dominant animal host and most of Yersinia enterocolitica strains isolated from it are pathogenic.It should be strengthened the monitoring, quarantine and management of swine in the future.