聚左旋乳酸/壳聚糖纳米纤维三维多孔支架复合骨髓间充质干细胞修复兔骨缺损**△

背景：骨髓间充质干细胞具有向多种间质细胞谱系分化的能力,且支架材料的性能对骨缺损的修复有重要影响。 目的：观察聚左旋乳酸/壳聚糖纳米纤维三维多孔支架复合骨髓间充质干细胞治疗骨缺损。 方法：对骨缺损模型兔分别采用空白植入、髂后上棘自体松质骨移植、聚左旋乳酸/壳聚糖纳米纤维多孔支架移植和复合了骨髓间充质干细胞的聚左旋乳酸/壳聚糖纳米纤维多孔支架移植修复缺损部位。 结果与结论：至移植12周,移植复合了骨髓间充质干细胞的聚左旋乳酸/壳聚糖纳米纤维多孔支架的实验兔的缺损处有骨组织生成,支架材料降解,已完成缺损修复,其修复情况接近松质骨组;髂后上棘自体松质骨移植的实验兔的缺损修复完好,新形成的骨组织较规则;只植入聚左旋乳酸/壳聚糖纳米纤维多孔支架的实验兔有少量骨组织形成,材料部分降解;空白植入的实验兔缺损处无新生骨组织生成,主要由纤维结缔组织填充。说明新型的生物支架材料聚左旋乳酸/壳聚糖纳米纤维三维多孔支架与来源于新西兰大白兔的骨髓间充质干细胞复合培养后,植入同种异体兔股骨髁缺损处,使骨缺损的修复速度加快,表现为较好的体内诱导成骨的作用。

Three-dimensional porous poly(L-lactic acid)/chitosan nanofiber scaffolds combined with bone marrow mesenchymal stem cells in repairing of bone defects

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have the ability to differentiate to a variety of mesenchymal cell lineages, and the properties of scaffold material have a great impact on bone defects. OBJECTIVE: To observe the possibility of repairing bone defects by three-dimensional porous poly(L-lactic acid)/chitosan nanofiber (PLLA/CSNF) scaffolds combined with BMSCs. METHODS: The rabbit bone defect models were implanted with posterior superior iliac spine autogenous cancellous bone (cancellous bone group), PLLA/CSNF porous scaffold and the PLLA/CSNF porous scaffold combined with BMSCs (BMSCs+PLLA/CSNF group). The model group was without implantation.    RESULTS AND CONCLUSION: Bone tissue was formed in the defected position in the BMSCs+PLLA/CSNF group after implanted for 12 weeks, the scaffolds were degraded and the repair of the defect was completed, the effect of repairing defects was similar to that in the cancellous bone group; in the cancellous bone group, the defects were healing well and the newly formed bone tissue were arranged in order; some new bone tissue were formed and the scaffolds were degraded partly in PLLA/CSNF group while the defect was mainly filled with fibrous connective tissue in model group and there was no new bone tissue formation. The three-dimensional porous PLLA/CSNF scaffolds co-cultured with BMSCs implanted into the defects of the femoral condyle could increase the speed of repairing and possesses a superior osteoinductivity in vivo.