子宫内膜癌患者FHIT、SLIT2、EDNRB基因杂合性丢失

目的：探讨子宫内膜癌患者FHIT、SLIT2、EDNRB基因3个微卫星位点D3S1287、 D4S1593、D13S160杂合性丢失（loss of heterozygosity，LOH），以确定侯选的抑癌基因。方法：应用PCR-变性 PAGE-银染方法分别对35例子宫内膜癌患者癌组织及相对应的正常子宫内膜组织在FHIT、SLIT2、EDNRB基因3个微卫星位点D3S1287、D4S1593、D13S160 行LOH检测。结果：LOH总检出率为54.3%，D3S1287、D4S1593、D13S160位点分别为34.5%、20.5%、19.3%。FHIT、SLIT2、EDNRB基因的3个微卫星位点发生LOH率与子宫内膜癌手术-病理分期无明显相关性。结论：子宫内膜癌患者肿瘤组织在FHIT、SLIT2、及EDNRB基因的微卫星位点D3S1287、D4S1593、D13S160 均有LOH。FHIT、SLIT2及EDNRB基因为抑癌基因，其失活可能与子宫内膜癌的发生有关。

Heterozygosity loss at FHIT, SLIT2 and EDNRB gene in endometrical carcinoma

Objective: To study the loss of heterozygosity(LOH) at FHIT,SLIT2 and EDNRB gene at microsatellite markers D3S1287,D4S1593,and D13S160 in endometrial carcinoma.Methods: Tumor tissues and relatively normal tissues of thirty-five cases of endometrial carcinoma were examined by using PCR-denaturing PAGE-silver staining at microsatellite markers D3S1287,D4S1593,and D13S160.Results: The total LOH rate was 54.3%,and LOH rate of D3S1287, D4S1593,and D13S160 was 34.5%,20.5%,and 19.3%,respectively.The LOH rate at FHIT,SLIT2,and EDNRB gene did not positively correlate with the stage of clinical-pathogensis in endometrial carcinoma.Conclusion: LOH exists at micro-satellite markers of FHIT,SLIT2,and EDNRB gene in endometrial carcinoma,suggesting that FHIT,SLIT2,and EDNRB gene are tumor suppressor genes(TSGs) and the inactivity of TSGs maybe one of the reasons for carcinoma.