| 大麦花药离体诱变及铝胁迫下的培养反应 |
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| 引用本文： | 孙月芳,陆瑞菊,王亦菲,周润梅,黄剑华.大麦花药离体诱变及铝胁迫下的培养反应[J].核农学报,2005,19(2):95-98. |
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| 作者姓名： | 孙月芳 陆瑞菊 王亦菲 周润梅 黄剑华 |
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| 作者单位： | 上海市农业遗传育种重点实验室;上海市农业科学院生物技术研究中心,上海,201106 |
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| 基金项目： | 国家重点科技攻关项目(96-C01-01-02-14) |
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| 摘 要： | 2份大麦品系的离体麦穗经辐照处理后 ,其花药在铝胁迫培养基上诱导愈伤组织 ,愈伤组织在铝胁迫培养基上进行分化。试验结果表明 ,离体麦穗辐照处理显著降低花药离体培养的愈伤组织诱导率和而后的绿苗分化率 ,诱导培养基中的较高铝浓度胁迫降低愈伤组织诱导率 ,分化培养基中的铝胁迫降低绿苗分化率。辐照处理后花药在铝胁迫培养基上诱导形成愈伤组织并在铝胁迫培养基上分化形成绿苗。
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| 关 键 词： | 大麦 花药 60Co辐照 铝胁迫 绿苗 |
| 文章编号： | 1000-8551(2005)02-095-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2004年3月18日 |
RESPONSE OF ANTHER CULTRUE IN VITRO TO IRRADIATION OF SPIKE AND CULTURING WITH AL STRESS IN BARLEY |
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| SUN Yue-fang,LU Rui-ju,WANG Yi-fei,ZHOU Run-mei,HUANG Jian-hua.RESPONSE OF ANTHER CULTRUE IN VITRO TO IRRADIATION OF SPIKE AND CULTURING WITH AL STRESS IN BARLEY[J].Acta Agriculturae Nucleatae Sinica,2005,19(2):95-98. |
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| Authors: | SUN Yue-fang LU Rui-ju WANG Yi-fei ZHOU Run-mei HUANG Jian-hua |
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| Affiliation: | SUN Yue-fang 1,2 LU Rui-ju 1,2 WANG Yi-fei 1,2 ZHOU Run-mei 1,2 HUANG Jian-hua 1,2 |
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| Abstract: | Spikes of two barely lines treated with 60 Co γ-radiation as anther culture materials, the callus induction and green plant regeneration were investigated on the media containing Al. The results showed that the frequency of callus induction was dramatically decreased by irradiation treatment and green plant regeneration was failed at a dose of 10 Gy. The calli was obtained from anthers cultured with Al-medium, and also green plants were regenerated even in higher Al concentration. The frequency of callus induction was reduced in culturing with higher stress of Al, and the green plant regeneration was also decreased. |
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| Keywords: | barely anther " target="_blank">60Coγ-irradiation zz')" href="#"> 60Coγ-irradiation Al stress green plant |
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