血管紧张素原M235T基因多态性对血压影响的研究

目的：研究血管紧张素原基因（ＡＧＴ）第二外显子Ｍ２３５Ｔ等位基因的多态性与高血压之间的关系。方法： 应用多聚酶链反应（ＰＣＲ）结合限制性酶切方法对１０５例健康体检者与１０２例原发性高血压（ＥＨ）患者进行基因突变的检测。结果：（１）ＥＨ患者Ｔ２３５ 等位基因频率（０．４４５）高于对照组（０．３２３），Ｐ＜ ０．０５。在男性ＥＨ患者与男性对照组中差别更为明显（Ｐ＜ ０．０１）；（２）在有家族史的ＥＨ 患者中，Ｍ２３５Ｔ 突变基因型（ＴＴ型）频率高于正常对照组（４２．１％ ＶＳ１８．８％ ，Ｐ＜０．０５）。结论：（１）ＡＧＴ基因的突变与ＥＨ的发病具有相关性。对男性ＥＨ影响可能更大；（２）在有家族史的高血压患者中， ＡＧＴ２３５的ＴＴ基因型与ＥＨ有关

The Influence of M235T Allele Polymorphism on Blood Pressure

Objectives: To study the association between M235T allele polymorphism in exon 2 of angiotensinogen(AGT) gene and essential hypertension(EH). Methods: Polymerase chain reaction combined with restrict enzyme digestion was used to detect the target gene variation in 105 normotensive controls and 102 hypertensive patients. Results:(1)T235 allele frequency was significantly higher in hypertensive patients than in controls and it was noticeably higher in male hypertensives;(2)Among the EH patients with family history, the frequency of T235 genotype (42.1% vs 18.8%) were higher in EH than in controls. Conclusion:(1)The M235T allele variation was associated with EH,especially in male;(2)The T235 genotype were associated with EH with family history