| 皂荚多糖超声波提取工艺优化及体外抗氧化活性研究 |
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| 引用本文： | 高洁,董文宾,王勇,张泉荣,张文秀.皂荚多糖超声波提取工艺优化及体外抗氧化活性研究[J].食品工业科技,2019,40(21):124-129,135. |
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| 作者姓名： | 高洁 董文宾 王勇 张泉荣 张文秀 |
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| 作者单位： | 1. 陕西国际商贸学院医药学院, 陕西西安 710000;2. 陕西科技大学, 陕西西安 710000;3. 陕西省食品加工工程技术研究中心, 陕西西安 710000;4. 陕西农产品加工技术研究院, 陕西西安 710000 |
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| 基金项目： | 陕西省大学生创新创业训练计划项目(201849005)陕西省教育厅科研计划项目(18JK0949)陕西国际商贸学院校级科研项目(SMXY201803)。国家级大学生创新创业训练计划项目(201849001) |
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| 摘 要： | 以皂荚多糖为研究对象,在单因素实验的基础上,采用响应面法对皂荚多糖的超声波提取工艺进行优化,并对多糖进行体外抗氧化活性的研究。结果表明:最佳提取工艺条件为:提取温度50 ℃,液料比35:1 (mL/g),提取时间30 min,超声功率285 W,提取3次,多糖得率为30.65%±0.25%。抗氧化实验表明,皂荚多糖具有一定的抗氧化能力,其对超氧阴离子自由基、DPPH自由基、羟自由基清除作用的IC50分别为:10.3、6.9、1.5 mg/mL。
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| 关 键 词： | 皂荚 多糖 抗氧化 超声波提取 |
| 收稿时间： | 2019-01-24 |
Optimization of Ultrasonic Assisted Extraction and Antioxidant Activity of Polysaccharide from Gleditsia japonic Miq. |
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| GAO Jie,DONG Wen-bin,WANG Yong,ZHANG Quan-rong,ZHANG Wen-xiu.Optimization of Ultrasonic Assisted Extraction and Antioxidant Activity of Polysaccharide from Gleditsia japonic Miq.[J].Science and Technology of Food Industry,2019,40(21):124-129,135. |
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| Authors: | GAO Jie DONG Wen-bin WANG Yong ZHANG Quan-rong ZHANG Wen-xiu |
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| Affiliation: | 1. College of Medical, Shanxi Institute of International Trade & Commerce, Xi'an 710000, China;2. Shanxi University of Science & Technology, Xi'an 710000, China;3. Shaanxi Research Institute of Food Processing Technology, Xi'an 710000, China;4. Shaanxi Research Institute of Agricultural Products Processing Technology, Xi'an 710000, China |
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| Abstract: | The polysaccharide in Gleditsia japonic Miq. was researched. On the basis of single factor experiment,response surface methodology was used to optimize the ultrasonic extraction process of Gleditsia japonic Miq.,and the antioxidant activity of polysaccharides in vitro was studied. The results showed that the optimum technology conditions were as follows:Extraction temperature 50 ℃,liquid to material ratio 35:1 (mL/g),extraction time 30 min,ultrasonic power 285 W,performed three times,under these conditions,the yield of polysaccharide was 30.65%±0.25%. Antioxidant activity test showed that the polysaccharide in Gleditsia japonic Miq.had certain antioxidant activity,and the IC50 of radical scavenging capacity on O2-·,DPPH· and ·OH were 10.3,6.9,1.5 mg/mL,respectively. |
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