| Similarity of Bovine Rotavirus Receptor and Human Rotavirus Receptor |
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| 作者姓名： | 苏琦华 訾自强 潘菊芬 徐燕 |
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| 作者单位： | Department of Microbiology，Tianjin Medical University，Department of Microbiology，Tianjin Medical University，Department of Immunology，Tianjin Medical University，Department of Microbiology，Tianjin Medical University Tianjin 300070，China，Tianjin 300070，China，Tianjin 300070，China，Tianjin 300070，China |
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| 摘 要： | Rotavirusesaretheleadingcauseofviralgastroenteritis worldwide.Theseviruses,includinghumanrotavirus (HRV)andbovinerotavirus(BRV),whichprimarilyin fectchildrenandyounganimals,cancauseseveredehy dratingdiarrhearesultinginsignificantmorbidity,mortali tyand…
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| 关 键 词： | 牛 轮状病毒受体 人类轮状病毒 病例报告 HRV 抗体 |
Similarity of Bovine Rotavirus Receptor and Human Rotavirus Receptor |
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| Qihua SU,Ziqiang ZI,Jufen PAN,and Yan XU.Similarity of Bovine Rotavirus Receptor and Human Rotavirus Receptor[J].Chinese Journal of Microbiology and Immunology,2004,2(1):24-28. |
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| Authors: | Qihua SU Ziqiang ZI Jufen PAN and Yan XU |
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| Affiliation: | [1]DepartmentofMicrobiology,TianjinMedicalUniversity,Tianjin300070,China [2]DepartmentofMicrobiology,TianjinMedicalUniversity,Tianjin300070,China [3]DepartmentofImmunology,TianjinMedicalUniversity,Tianjin300070,China |
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| Abstract: | The monoclonal antibody against bovine rotavirus (BRV) receptor (BRV-R-mAb) was used to explore the similarity between the receptors of BRV and human rotavirus (HRV). ELISA, dot immunobinding assay, cell protection assay, solid-phase assay and immunohistochemistry method were applied. BRV-R-mAb bound both anti-BRV IgG and anti-HRV IgG respectively and could protect MA 104 cells against BRV and HRV challenges. Immunohistochemistry test showed that there were rotavirus receptors on the surfaces of foetal intestinal, tracheal mucosa and MA 104 cells membrane. We purified the rotavirus receptors on MA 104 cells, which could bind both BRV and HRV in vitro. It is concluded that BRV receptor and HRV receptor are homogenous proteins and can be recognized by both BRV and HRV. |
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| Keywords: | Monoclonal antibody Receptors of rotavirus Homogenous proteins |
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