Mechanism of action of relaxant effect of Agastache mexicana ssp.mexicana essential oil in guinea-pig trachea smooth muscle |
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Authors: | Andrés Navarrete Natalia Ávila-Rosas Mateo Majín-León José Luis Balderas-López Alejandro Alfaro-Romero José Carlos Tavares-Carvalho |
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Affiliation: | 1. Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México Ciudad Universitaria, Coyoacán, Ciudad de México, México;2. Laboratório de Pesquisa em Fármacos, Curso de Farmácia, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá, Macapá, AP, Brazil |
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Abstract: | Context: Agastache mexicana ssp. mexicana (Kunth) Lint &; Epling (Lamiaceae), popularly known as ‘toronjil morado’, is used in Mexican traditional medicine for the treatment of several diseases such as hypertension, anxiety and respiratory disorders.Objective: This study investigates the relaxant action mechanism of A. mexicana ssp. mexicana essential oil (AMEO) in guinea-pig isolated trachea model.Materials and method: AMEO was analyzed by GC/MS. The relaxant effect of AMEO (5–50?μg/mL) was tested in guinea-pig trachea pre-contracted with carbachol (3?×?10???6?M) or histamine (3?×?10???5?M) in the presence or absence of glibenclamide (10???5?M), propranolol (3?×?10???6?M) or 2′,5′-dideoxyadenosine (10???5?M). The antagonist effect of AMEO (10–300?μg/mL) against contractions elicited by carbachol (10???15–10???3?M), histamine (10???15–10???3?M) or calcium (10–300?μg/mL) was evaluated.Results: Essential oil composition was estragole, d-limonene and linalyl anthranilate. AMEO relaxed the carbachol (EC50?=?18.25?±?1.03?μg/mL) and histamine (EC50?=?13.3?±?1.02?μg/mL)-induced contractions. The relaxant effect of AMEO was not modified by the presence of propranolol, glibenclamide or 2′,5′-dideoxyadenosine, suggesting that effect of AMEO is not related to β2-adrenergic receptors, ATP-sensitive potassium channels or adenylate cyclase activation. AMEO was more potent to antagonize histamine (pA2′?=??1.507?±?0.122) than carbachol (pA2′?=??2.180?±?0.357). Also, AMEO antagonized the calcium chloride-induced contractions.Conclusion: The results suggest that relaxant effect of AMEO might be due to blockade of calcium influx in guinea-pig trachea smooth muscle. It is possible that estragole and d-limonene could contribute majority in the relaxant effect of AMEO. |
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Keywords: | Estragole d-limonene" target="_blank">d-limonene asthma pA2′ airways |
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