不同体质MRL／lpr狼疮鼠B淋巴细胞刺激因子及其受体基因及蛋白质的表达

目的：建立MRL／lpr狼疮鼠体质的区分方法，并于不同周龄观察肾脏B淋巴细胞刺激因子（ BAFF）、B淋巴细胞刺激因子受体（ BAFF-R）基因及蛋白质的表达，以考察狼疮鼠体质差异的物质基础。方法将12周龄MRL／lpr小鼠按体质分为寒体质、常体质及热体质，随机选取不同体质的小鼠各20只为寒体组、热体组、常体组。分别于12周龄时及20周龄时取材。通过RT-PCR、West-ern Blot等检测方法，检测肾脏 BAFF mRNA、BAFF-R mRNA 及其蛋白质表达。结果寒体质MRL／lpr狼疮鼠肾组织BAFF mRNA及蛋白质、BAFF-R蛋白质的表达均较常体质、热体质低，但各组BAFF-R mRNA的表达无明显差异。结论 MRL／lpr狼疮鼠体质差异可能与肾脏组织BAFF mRNA及蛋白质、BAFF-R蛋白质的表达有关。

Gene and protein expression of B cell-activating factor and its receptor in MRL/lpr lupus mice with different constitutions

Objective To establish the method to distinguish the constitution of MRL/lpr lupus mice and to study the gene and protein expression of renal B cell-activating factor ( BAFF) , B cell stimulating fac-tor receptor ( BAFF-R) , so as to reveal the factors at molecular biological level leading to the differences of constitution.Methods Firstly, MRL/lpr female mice of 12-week age were classified into cold consti-tution, heat constitution and normal constitution.Secondly, 20 mice were randomly selected respectively from cold constitution group, heat constitution group and normal constitution group.Thirdly, every 10 mice in each group were sacrificed at 12-week age and at 20-week age respectively.Then, expressions of renal BAFF mRNA, BAFF-R mRNA, BAFF protein and BAFF-R protein were examined by real-time PCR and Western Blot.Results Expressions of BAFF mRNA ,BAFF protein and BAFF-R protein were the lowest in the cold constitution group of all the groups.The expression of BAFF-R mRNA showed no significant difference among all groups.Conclusion The constitutional differences could be related to the expressions of renal BAFF mRNA ,BAFF protein and BAFF-R protein .